Home
  >  
Section 10
  >  
Chapter 9,585

The human estrogen receptor hormone binding domain dimerizes independently of ligand activation

Salomonsson, M.; Häggblad, J.; O'Malley, B.W.; Sitbon, G.M.

Journal of Steroid Biochemistry and Molecular Biology 48(5-6): 447-452

1994

ISSN/ISBN: 0960-0760
PMID: 8180105
DOI: 10.1016/0960-0760(94)90192-9
Accession: 009584345
Download citation:  
Text
  |  
BibTeX
  |  
RIS
High level expression of biochemically active human estrogen receptor hormone binding domain (hER-HBD) was achieved using a Saccharomyces cerevisiae expression system. Using dissociation kinetic analysis, density gradient centrifugation and cross-linking studies, a spontaneous dimerization activity of hER-HBD independent of the presence of the DNA binding domain, ligand, and of elevated temperature is demonstrated.

PDF emailed within 0-6 h: $19.90




Related References

Kallen, J.; Schlaeppi, J.M.; Bitsch, F. 2004: Evidence for Ligand-independent Transcriptional Activation of the Human Estrogen-related Receptor a (ERRa). Crystal Structure of ERRa Ligand Binding Domain in Complex with Peroxisome Proliferator-activated Receptor Coactivor-1a Journal of Biological Chemistry 279(47): 330-337
Kallen, J.; Schlaeppi, J-Marc.; Bitsch, F.; Filipuzzi, I.; Schilb, A.; Riou, V.; Graham, A.; Strauss, A.; Geiser, M.; Fournier, B. 2004: Evidence for ligand-independent transcriptional activation of the human estrogen-related receptor alpha (ERRalpha): crystal structure of ERRalpha ligand binding domain in complex with peroxisome proliferator-activated receptor coactivator-1alpha Journal of Biological Chemistry 279(47): 49330-49337
Reese, J.C.; Katzenellenbogen, B.S. 1991: Mutagenesis of cysteines in the hormone binding domain of the human estrogen receptor. Alterations in binding and transcriptional activation by covalently and reversibly attaching ligands Journal of Biological Chemistry 266(17): 10880-10887
Lee, H-Seog.; Miyauchi, K.; Nagata, Y.; Fukuda, R.; Sasagawa, S-ichi.; Endoh, H.; Kato, S.; Horiuchi, H.; Takagi, M.; Ohta, A. 2002: Employment of the human estrogen receptor beta ligand-binding domain and co-activator SRC1 nuclear receptor-binding domain for the construction of a yeast two-hybrid detection system for endocrine disrupters Journal of Biochemistry 131(3): 399-405
Reese, J.C.; Katzenellenbogen, B.S. 1992: Characterization of a temperature-sensitive mutation in the hormone binding domain of the human estrogen receptor : Studies in cell extracts and intact cells and their implications for hormone-dependent transcriptional activation The Journal of Biological Chemistry 267(14): 9868-9873
Reese, J.C.; Katzenellenbogen, B.S. 1992: Characterization of a temperature-sensitive mutation in the hormone binding domain of the human estrogen receptor. Studies in cell extracts and intact cells and their implications for hormone-dependent transcriptional activation Journal of Biological Chemistry 267(14): 9868-9873
Dunn, C.A.; Clark, W.; Black, E.J.; Gillespie, D.A.F. 2003: Estrogen receptor activation function 2 (AF-2) is essential for hormone-dependent transactivation and cell transformation induced by a v-Jun DNA binding domain-estrogen receptor chimera Biochimica et Biophysica Acta 1628(3): 147-155
Webster, N.J.; Green, S.; Tasset, D.; Ponglikitmongkol, M.; Chambon, P. 1989: The transcriptional activation function located in the hormone-binding domain of the human oestrogen receptor is not encoded in a single exon EMBO Journal 8(5): 1441-1446
Ekena, K.; Weis, K.E.; Katzenellenbogen, J.A.; Katzenellenbogen, B.S. 1996: Identification of amino acids in the hormone binding domain of the human estrogen receptor important in estrogen binding Journal of Biological Chemistry 271(33): 20053-20059
Reese, J.C.; Wooge, C.H.; Katzenellenbogen, B.S. 1992: Identification of two cysteines closely positioned in the ligand-binding pocket of the human estrogen receptor: roles in ligand binding and transcriptional activation Molecular Endocrinology 6(12): 2160-2166
Kao, H.Y.; Han, C.C.; Komar, A.A. 2003: Co-repressor Release but Not Ligand Binding Is a Prerequisite for Transcription Activation by Human Retinoid Acid Receptor a Ligand-binding Domain The Journal of Biological Chemistry 278(9): 66-73
Kao, H.-Y.; Han, C.C.; Komar, A.A.; Evans, R.M. 2003: Co-repressor release but not ligand binding is a prerequisite for transcription activation by human retinoid acid receptor alpha ligand-binding domain Journal of Biological Chemistry 278(9): 7366-7373
Beekman, J.M.; Allan, G.F.; Tsai, S.Y.; Tsai, M.J.; O'Malley, B.W. 1993: Transcriptional activation by the estrogen receptor requires a conformational change in the ligand binding domain Molecular Endocrinology 7(10): 1266-1274
Valentine, J.E.; Kalkhoven, E.; White, R.; Hoare, S.; Parker, M.G. 2000: Mutations in the estrogen receptor ligand binding domain discriminate between hormone-dependent transactivation and transrepression Journal of Biological Chemistry 275(33): 25322-25329
Lee, J.W.; Mcfadden, M.C.; Macy, B.; Moore, D.D.; Heyman, R.A. 1994: The ligand binding domain of the thyroid hormone receptor requires retinoid X receptor for full transcriptional activation function in yeast Journal of Cellular Biochemistry Suppl 1994(18B): 345