Section 10
Chapter 9,585

The human estrogen receptor hormone binding domain dimerizes independently of ligand activation

Salomonsson, M.; Häggblad, J.; O'Malley, B.W.; Sitbon, G.M.

Journal of Steroid Biochemistry and Molecular Biology 48(5-6): 447-452


ISSN/ISBN: 0960-0760
PMID: 8180105
DOI: 10.1016/0960-0760(94)90192-9
Accession: 009584345

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High level expression of biochemically active human estrogen receptor hormone binding domain (hER-HBD) was achieved using a Saccharomyces cerevisiae expression system. Using dissociation kinetic analysis, density gradient centrifugation and cross-linking studies, a spontaneous dimerization activity of hER-HBD independent of the presence of the DNA binding domain, ligand, and of elevated temperature is demonstrated.

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