Section 10
Chapter 9,585

The human leukotriene A4 hydrolase gene is expressed in two alternatively spliced mRNA forms

Jendraschak, E.; Kaminski, W.E.; Kiefl, R.; von Schacky, C.

Biochemical Journal 314: 733-737


ISSN/ISBN: 0264-6021
PMID: 8615763
Accession: 009584551

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We identified a novel short (s-) mRNA of the human leukotriene-A-4 (LTA-4) hydrolase using sequential reverse transcriptase PCR mapping. The s-mRNA is generated by skipping an 83 hp exon located in the 3' coding region and suggests the expression of an LTA-4 hydrolase isoform with a calculated molecular mass of 59 kDa and a distinct C-terminus. Both LTA-4 hydrolase mRNAs are constitutively expressed in blood cells, endothelial cells, smooth muscle cells, fibroblasts and tumour cells. The ratios of their mRNA expression levels are cell specific, with relatively high s-form mRNA expression in reticulocytes. Our data strongly suggest that the novel mRNA codes for the structurally related but distinct LTA-4 hydrolase isoenzyme that has been postulated.

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