+ Site Statistics
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn

+ Translate
+ Recently Requested

The incorporation of dietary n-3 polyunsaturated fatty acids into porcine platelet phospholipids and their effects on platelet thromboxane A-2 release

The incorporation of dietary n-3 polyunsaturated fatty acids into porcine platelet phospholipids and their effects on platelet thromboxane A-2 release

Prostaglandins Leukotrienes & Essential Fatty Acids 56(3): 223-228

We investigated the dynamic changes in fatty acid (FA) composition in platelet phospholipid (PL) and in the release of thromboxane from stimulated platelets in pigs prefed a diet enriched with polyunsaturated fatty acids (PUFA) of the n-3 and n-6 families of FAs. For 20 days, we fed pigs diets supplemented with either corn oil, fish oil, or fish and borage oil. Platelets were separated from blood drawn at baseline and then on day 4, 8, 12, 16 and 20 of the dietary regimen. Analysis of the FA contained in platelet PL demonstrated that significant changes in PUFA composition occurred during dietary supplementation with fish oil and fish and borage oil. Eicosapentaenoic acid (EPA) from fish oil was rapidly incorporated into PL of platelets and reached a steady-state plateau by day 12. An increase in the content of docosahexaenoic acid (DHA) in the platelets' PL was observed in both groups receiving fish oil. This increase in n-3 PUFA in platelet PL occurred with a concomitant decrease in the content of n-6 FA, primarily of arachidonic acid (AA) in the PL of platelets in both fish oil groups. Dietary supplementation with borage oil, which contains gammalinolenic acid (GLA), increased dihomogammalinolenic acid (DGLA) content in platelet PL. Following A-23187 stimulation of platelets, levels of thromboxane B-2 (TxB-2), the stable metabolite of thromboxane A-2 (TxA-2), were significantly suppressed in both fish oil groups compared to the corn oil-supplemented group. Dietary supplementation of EPA and EPA plus GLA increased platelet PL content of EPA, and EPA and DGLA, respectively. Following platelet stimulation, TxA-2 production was significantly increased, an increase that was attenuated in the platelets from pigs prefed fish oil, and even more so in the fish and borage-oil-prefed pigs.

(PDF emailed within 0-6 h: $19.90)

Accession: 009588140

Download citation: RISBibTeXText

PMID: 9089803

DOI: 10.1016/s0952-3278(97)90539-1

Related references

Effect of dietary n 3 and n 6 polyunsaturated fatty acids pufa on platelet function and platelet phospholipids in atherosclerotic swines. Journal of Molecular & Cellular Cardiology 23(SUPPL 5): S89, 1991

Effects of diets high in saturated fatty acids, canola oil, or safflower oil on platelet function, thromboxane B2 formation, and fatty acid composition of platelet phospholipids. American Journal of Clinical Nutrition 54(2): 351-358, 1991

Effects of the incubation of long-chain polyunsaturated fatty acids on platelet lipids and thromboxane release. Prostaglandins Leukotrienes & Essential Fatty Acids 56(4): 259-264, 1997

Effects of dietary fatty acids on platelet aggregation and platelet thromboxane b 2 production in the rabbit. Thrombosis & Haemostasis 42(1): 405, 1979

A comparison of the effects of dietary short and long chain saturated fatty acids on platelet functions, platelet phospholipids, and blood coagulation in rats. Laboratory Investigation; a Journal of Technical Methods and Pathology 43(5): 438-442, 1980

Platelet-aggregating effects of platelet-activating factor-like phospholipids formed by oxidation of phosphatidylcholines containing an sn-2-polyunsaturated fatty acyl group. Biochimica et Biophysica Acta 1210(2): 202-208, 1994

Comparative aspects of omega-3 and omega-6 polyunsaturated fatty acids in rabbits and rats: effects on platelet function, thromboxane and prostacyclin generation, tissue phospholipid fatty acids and membrane fluidity. British Journal of Clinical Practice. Supplement 31: 37-41, 1984

Selective incorporation of dietary 3 polyunsaturated fatty acids into rat cerebellar phospholipids. Bulletin of Experimental Biology and Medicine 106(6): 1714-1717, 1988

Effects of dietary supplementation with pyridoxal 5'-phosphate and polyunsaturated fatty acids on rat platelet aggregation. Biochemical Society Transactions 16(3): 385-386, 1988

Effects of dietary fatty acids on platelet plat function plasma thromboxane b 2 txb 2 levels and plat phospholipid pl fatty acids. FASEB Journal 4(3): A780, 1990

Hypocoagulant and lipid-lowering effects of dietary n-3 polyunsaturated fatty acids with unchanged platelet activation in rats. Arteriosclerosis, Thrombosis, and Vascular Biology 18(9): 1480-1489, 1998

Dietary n-9, n-6, and n-3 fatty acids modify linoleic acid more than arachidonic acid levels in plasma and platelet lipids and minimally affect platelet thromboxane formation in the rabbit. Journal of Nutritional Biochemistry 1(11): 565-571, 1990

Magnesium deprivation or short-term essential fatty acid deficiency in rats: effects on serum lipids, platelet fatty acid composition and arachidonic acid incorporation into platelet phospholipids. Magnesium Research 1(1-2): 39-44, 1988

Effects of dietary n-3 polyunsaturated fatty acids from plant and marine origin on platelet aggregation in healthy elderly subjects. British Journal of Nutrition 82(3): 183-191, 2000

Platelet functions and fatty acid composition of platelet phospholipids in spontaneously hypertensive rats fed saturated or polyunsaturated fats. Atherosclerosis 38(1-2): 129-136, 1981