EurekaMag.com logo
+ Site Statistics
References:
53,869,633
Abstracts:
29,686,251
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on LinkedInFollow on LinkedIn

+ Translate

The major 20-kDa polysaccharide of Staphylococcus epidermidis extracellular slime and its antibodies as powerful agents for detecting antibodies in blood serum and differentiating among slime-positive and -negative S. epidermidis and other staphylococci species



The major 20-kDa polysaccharide of Staphylococcus epidermidis extracellular slime and its antibodies as powerful agents for detecting antibodies in blood serum and differentiating among slime-positive and -negative S. epidermidis and other staphylococci species



Archives of Biochemistry and Biophysics 342(2): 389-395



Staphylococcus epidermidis has been recognized as an important pathogen in immunocompromised hosts and patients with prosthetic or implanted medical devices. A highly adhesive extracellular material (slime or biofilm) produced by certain strains is associated with bacterial adherence to and growth on biomaterials contributing to pathogenesis of bacteremia. We have recently reported on the isolation and characterization of a sulfated 20-kDa acidic polysaccharide which constitutes slime's major component. Immunization of rabbits with crude slime and 20-kDa polysaccharide gave rise to readily reactive sera without manipulation of the 20-kDa polysaccharide structure. Immunological studies using purified polyclonal antibodies to 20-kDa polysaccharide by direct and competitive ELISA showed that they exhibit a high degree of reactivity and specificity with the homologous antigen. A significant proportion of the reactivity of antibodies to crude slime was also shown to be attributed to the 20-kDa polysaccharide. This polysaccharide is immunogenic in humans since blood sera derived from patients 10-15 days after confirmation of slime-producing S. epidermidis bacteremia gave approximately 16 times higher reactivity than that of healthy individuals. Antibodies to 20-kDa polysaccharide were able to recognize and react specifically with slime-positive S. epidermidis strains compared to slime-negative ones (2 to 5 times higher reactivity). Moreover, these antibodies exhibited statistically significant (P < 0.05) differences in the degree of reactivity among S. epidermidis and other staphylococci species. These results open a new area in the diagnosis of S. epidermidis infection by direct analysis in blood sera, in differentiating among slime-positive and slime-negative strains as well as in distinguishing slime-producing S. epidermidis from other staphylococci species by simple laboratory tests.

(PDF emailed within 0-6 h: $19.90)

Accession: 009596907

Download citation: RISBibTeXText

PMID: 9186502

DOI: 10.1006/abbi.1997.0107



Related references

Ultrastructural analysis of slime positive & slime negative Staphylococcus epidermidis isolates in infectious keratitis. Indian Journal of Medical Research 125(6): 767-771, 2007

Ultrastructural analysis of slime positive & slime negative Staphylococcus epidermidis isolates in infectious keratitis. Indian Journal of Medical Research 125(6): 767-771, 2007

Adherence to and accumulation of S epidermidis on different biomaterials due to extracellular slime production In vitro comparison of a slime-producing strain and its isogenic slime negative mutant. Zentralblatt fuer Bakteriologie 289(3): 355-364, July, 1999

Adherence to and accumulation of S. epidermidis on different biomaterials due to extracellular slime production: In vitro comparison of a slime-producing strain (Rp 62 A) and its isogenic slime negative mutant (M7). Zentralblatt fuer Bakteriologie 289(3): 355-364, 1999

Isolation and characterization of a novel 20-kDa sulfated polysaccharide from the extracellular slime layer of Staphylococcus epidermidis. Archives of Biochemistry and Biophysics 308(2): 432-438, 1994

Affinity and avidity of polyclonal antibodies towards the major antigenic determinant of slime-producing Staphylococcus epidermidis and their protective effect in experimental keratitis. Abstracts of the General Meeting of the American Society for Microbiology 102: 11-12, 2002

Levels of specific antibodies towards the major antigenic determinant of slime-producing Staphylococcus epidermidis determined by an enzyme immunoassay and their protective effect in experimental keratitis. Journal of Pharmaceutical & Biomedical Analysis 29(1-2): 255-262, 30 June, 2002

Predicting the pathogenicity of coagulase negative staphylococcus in the neonate slime production antibiotic resistances and predominance of staphylococcus epidermidis species. Pediatric Research 20(4 PART 2): 397A, 1986

In vitro activity of fleroxacin and 14 other antimicrobials against slime- and non-slime-producing Staphylococcus epidermidis. ChemoTherapy 35(5): 351-354, 1989

Unique properties of staphylococcus epidermidis extractable polysaccharide slime seep. Clinical Research 36(3): 455A, 1988

The role of extracellular slime in opsonophagocytosis of Staphylococcus epidermidis. Journal of Medical Microbiology 27(3): 207-213, 1988

Description of a slime negative mutant of staphylococcus epidermidis. Abstracts of the Annual Meeting of the American Society for Microbiology 90: 99, 1990

Immunochemical analysis of the extracellular slime substance of Staphylococcus epidermidis. European Journal of Clinical Microbiology & Infectious Diseases 9(4): 262-270, 1990

Investigation on extracellular slime substance produced by Staphylococcus epidermidis. Zentralblatt für Bakteriologie, Mikrobiologie, und Hygiene. Series A, Medical Microbiology, Infectious Diseases, Virology, Parasitology 258(2-3): 256-267, 1984

Extracellular slime substance as a virulence determinant in Staphylococcus epidermidis. Zentralblatt fuer Bakteriologie Supplement 27(0): 109-116, 1994