+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

The molecular genetics of feline coronaviruses: comparative sequence analysis of the ORF7a/7b transcription unit of different biotypes

The molecular genetics of feline coronaviruses: comparative sequence analysis of the ORF7a/7b transcription unit of different biotypes

Virology 212(2): 622-631

Feline coronaviruses (FCoVs) have been subdivided into feline enteric coronaviruses (FECVs) and feline infectious peritonitis viruses (FIPVs) on the basis of pathogenic properties. Serologically, a distinction has been made between type I and II FCoVs, the latter of which more closely resemble canine coronavirus (CCV). To gain more insight into the genetic relationships between different FCoV biotypes, we determined the nucleotide sequences of the ORF7a/7b transcription unit of nine strains. The following observations were made: (i) The sequences are 87-100% identical. In this part of the genome, type I and II FCoVs are more closely related to each other than to CCV. To explain the genetic and antigenic differences between the spike genes of type I and II FCoVs, we postulate that type II FcoVs have arisen by an RNA recombination event between a type I FCoV and CCV. (ii) The avirulent "FECV" strains UCD and 79-1683 are more similar to virulent "FIPV" strains than to each other. Our findings thus support the notion that FECV and FIPV are not different species but merely virulence variants. (iii) In contrast to FECV 79-1683, FECV UCD contains an intact ORF7b, indicating that ORF7b deletions are not a universal distinguishing property of FECVs. (iv) ORF7b deletions readily occur in vitro, correlating with loss of virulence. By reverse transcription-polymerase chain reaction analysis, we show that in naturally occurring FCoVs ORF7b is maintained. Thus, ORF7b seems to provide a distinct selective advantage during natural infection.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 009599880

Download citation: RISBibTeXText

PMID: 7571432

DOI: 10.1006/viro.1995.1520

Related references

Comparative biological and biochemical properties of the feline coronaviruses feline infectious peritonitis virus and feline enteric coronavirus. Journal Of Cellular Biochemistry Supplementrt D: 297, 1986

Cloning and sequence analysis of the spike gene from several feline coronaviruses. Advances in Experimental Medicine and Biology 342: 17-21, 1993

Sequence analysis of feline coronaviruses and the circulating virulent/avirulent theory. Emerging Infectious Diseases 17(4): 744-746, 2011

Sequence Analysis of Feline Coronaviruses and the Circulating Virulent/Avirulent Theory. Emerging Infectious Diseases 17(4): 744-746, 2011

Sequence analysis of the nucleocapsid gene of feline coronaviruses circulating in Italy. New Microbiologica 33(4): 387-392, 2010

Comparative in vivo analysis of recombinant type II feline coronaviruses with truncated and completed ORF3 region. Plos one 9(2): E88758, 2014

Comparison of the amino acid sequence and phylogenetic analysis of the peplomer, integral membrane and nucleocapsid proteins of feline, canine and porcine coronaviruses. Microbiology and Immunology 40(6): 425-433, 1996

Comparative properties of feline coronaviruses in vitro. Canadian Journal of Veterinary Research 51(2): 212-216, 1987

The molecular dynamics of feline coronaviruses. Veterinary Microbiology 69(1-2): 117-125, 1999

One-tube fluorogenic reverse transcription-polymerase chain reaction for the quantitation of feline coronaviruses. Journal of Virological Methods 77(1): 37-46, 1999

Reverse gyrase gene from Sulfolobus shibatae B12: gene structure, transcription unit and comparative sequence analysis of the two domains. Nucleic Acids Research 24(23): 4668-4675, 1996

Data regarding the molecular biology of feline coronaviruses. Magyar Allatorvosok Lapja 121(5): 292-297, 1999

Molecular interactions of group 1 coronaviruses with feline APN. Advances in Experimental Medicine and Biology 581: 289-291, 2006

Molecular approaches to the pathogenesis of feline coronaviruses. Molecular pathogenesis of virus infections: 125-136, 2005

Antigenic and biological diversity of feline coronaviruses: feline infectious peritonitis and feline enteritis virus. Archives of Virology 96(1-2): 29-38, 1987