EurekaMag.com logo
+ Site Statistics
References:
48,252,460
Abstracts:
22,798,453
+ Resolve Accession
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
Submit PDF Full TextSubmit PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

The orientation and dynamics of the C2'-OH and hydration of RNA and DNAcntdotRNA hybrids


, : The orientation and dynamics of the C2'-OH and hydration of RNA and DNAcntdotRNA hybrids. Nucleic Acids Research 26(13): 3104-3110, July 1

The stereochemical and dynamic properties of the C2' hydroxyl group in several DNAcntdotRNA hybrids have been measured by NMR and compared with the homologous RNA duplex. The C2'-OH NMR signals of the RNA strands were identified, and numerous specific assignments were made. The rate constants for exchange of the hydroxyl protons with water were determined at 5degreeC, and were found to depend on both the position within a particular sequence and the nature of the duplex. On average, the exchange rate constants were slowest for the hybrids of composition rRcntdotdY, and fastest for the RNA duplex, with an overall range of apprx10-50/s. In the DNAcntdotRNA hybrids, strong NOEs and ROEs were observed between the OH and the H1' of the same sugar, unambiguously showing that the OH proton points toward the H1' most of the time, and not toward the O3' of the same sugar. Evidence for significant hydration in both grooves of the DNAcntdotRNA hybrids and the DNA duplex was found in ROESY and NOESY experiments. On average, the minor groove of the DNAcntdotRNA hybrids showed more kinetically significant hydration than the DNA, which can be attributed to the hydrophilic lining of hydroxyl groups in RNA.

(PDF 0-2 workdays service)

Order PDF Full Text

Close
Close

Click here to order any other PDF Full Text


Accession: 009604305

PDF Full Text: Submit


Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:
Close
Close

Other references

Koletsky, S., 1973: Obese spontaneously hypertensive rats--a model for study of atherosclerosis. Experimental and Molecular Pathology 19(1): 53-60

Wang, H.; Xu, J.; Gao, Z.; Lu, C.; Yao, S.; Ma, J., 2016: An Event-Based Neurobiological Recognition System with Orientation Detector for Objects in Multiple Orientations. NlmCategory="UNASSIGNED">A new multiple orientation event-based neurobiological recognition system is proposed by integrating recognition and tracking function in this paper, which is used for asynchronous address-event representation...

Van Es, L., 1931: Remarks on Avian Tuberculosis Infection in Mammals. The author gives an account of the results obtained from the final experiments of an investigation which was commenced in 1928 to study the importance of avian tuberculous infection in cattle and human beings. Out of 105 cases in domesticated bird...

Johnson L.B.; Davis L.C.; Stuteville D.L., 1980: Ethane and ethylene evolution from alfalfa seedlings infected with peronospora trifoliorum. Shoots from healthy downy mildew-susceptible alfalfa [Medicago sativa] seedlings placed in glass syringes released small amounts of both ethane and ethylene during a 12-16 h incubation period in the dark. Susceptible shoots at 7 days after inocula...

Boyce Glenn M.; Abramson Lee W., 1992: Slope stabilization methods used on the Interstate Route H-3 Project. Proceedings of the Symposium on Engineering Geology and Geotechnical Engineering 28: 59-72

Radermacher, H; Schaloske, M., 1983: Problems of the water economy in Bahrain. Zeitschrift fur Bewasserungswirtschaft 18(1): 56-87

Chen, D..; Chen, G.., 1995: Investigations of entomogenous mermithid nematodes at rice fields in Zaoyang City Hubei Province. Journal of Central China Normal University (Natural Sciences). Mar; 291: 106-110 (Sum 73

Towery, R.B.ooke; Fawcett, N.C.; Zhang, P.; Evans, J.A., 2001: Genomic DNA hybridizes with the same rate constant on the QCM biosensor as in homogeneous solution. Hybridization rates of sheared, genomic E. coli DNA in 0.14 M, pH 6.7 phosphate buffer at 65degreeC were determined by: (1) observing the rate of absorbance decrease at 260 nm due to self-hybridization in solution; and (2) measurement of the rate...

Bell R.; Glade T., 2004: Quantitative risk analysis for landslides; examples from Bildudalur, NW-Iceland. Although various methods to carry out quantitative landslide risk analyses are available, applications are still rare and mostly dependent on the occurrence of disasters. In Iceland, two catastrophic snow avalanches killed 34 people in 1995. As a...

Heilmann, L., 1989: Clinical results after hemodilution with hydroxyethyl starch in pregnancy. Plasma volume contraction in pregnancy is diagnosed by an increase of hematocrit above 38%. Hydroxyethylstarch was administered to 30 patients with hemoconcentration alone, to 36 patients with fetal growth weight retardation and to two patients wi...