EurekaMag
+ Translate
+ Most Popular
The pigeon tick (Argas reflexus): its biology, ecology, and epidemiological aspects
Prevalence of hemoglobin abnormalities in Kebili (Tunisian South)
Lipogranuloma: a preventable complication of dacryocystorhinostomy
Value of basal plasma cortisol assays in the assessment of pituitary-adrenal insufficiency
Bees from the Belgian Congo. The acraensis group of Anthophora
Placing gingival retraction cord
Total serum IgE, allergy skin testing, and the radioallergosorbent test for the diagnosis of allergy in asthmatic children
Acariens plumicoles Analgesoidea parasites des oiseaux du Maroc
Injuries of terminal phalanges of the fingers in children
Biology of flowering and nectar production in pear (Pyrus communis)
Das Reliktvorkommen der Aspisviper (Vipera aspis L.) im Schwarzwald
Hydrological modelling of drained blanket peatland
Pathologic morphology and clinical significance of the anomalous origin of the left circumflex coronary artery from the right coronary artery. General review and autopsy analysis of 30 cases
Cyto genetic analyses of lymphocyte cultures after exposure to calcium cyclamate
Axelrodia riesei, a new characoid fish from Upper Rio Meta in Colombia With remarks concerning the genus Axelrodia and description of a similar, sympatric, Hyphessobrycon-species
Favorable evolution of a case of tuberculosis of pancreas under antibiotic action
RIFM fragrance ingredient safety assessment, Valencene, CAS Registry Number 4630-07-3
Parenteral microemulsions: an overview
Temperate pasture: management for grazing and conservation
Evaluation of a new coprocessed compound based on lactose and maize starch for tablet formulation
Thermal expansion and cracking of three confined water-saturated igneous rocks to 800C
Revision of the genera of the tribe Stigmoderini (Coleoptera: Buprestidae) a discussion of phylogenetic relationships
Anal tuberculosis. Report of a case
Gastric tuberculosis in the past and present
Adaptive responses of the cardiovascular system to prolonged spaceflight conditions: assessment with Holter monitoring

The predischarge chromophore in bacteriorhodopsin: A 15N solid-state NMR study of the L photointermediate


The predischarge chromophore in bacteriorhodopsin: A 15N solid-state NMR study of the L photointermediate



Biochemistry 36(31): 9316-9322



ISSN/ISBN: 0006-2960

PMID: 9235973

DOI: 10.1021/bi970416y

The L-550 intermediate in the bacteriorhodopsin (bR) photocycle has drawn much attention with respect to the mechanism of light-driven proton transport because it selectively releases the Schiff base (SB) proton to the extracellular proton channel in the L fwdarw M transition. Here we extend our solid-state NMR studies of bR photocycle intermediates to the L state. Under conditions that stabilize L-550, a new SB signal is detected in the 15N NMR spectrum which disappears upon thermal relaxation. This signal is in the range for a protonated SB, but downfield from the SB signals of bR-568 and N-520. Since steric interactions would have the opposite effect on shielding, the data argue against a 13,14-dicis chromophore in L-550. Comparison with the 15N chemical shifts of halide salts of protonated Schiff bases (pSB's) of retinal suggests that the interaction of the SB with its counterion is significantly stronger in L-550 than in N-520 (which in turn is stronger than in bR-568). This is consistent with models of the early photocycle in which the electrostatic interaction between the SB and its counterion constitutes an important constraint. Although the L counterion interaction is comparable to that of a 6-s-trans,13-cis chloride salt, the visible spectrum is strongly red-shifted from the lambda-max = 491 nm of the chloride. This suggests some double bond strain in L-550, particularly about the C dbd N bond. This strain is apparently gone in the N intermediate, which has a normal relationship between the 15N chemical shift and lambda-max. Such a relaxed chromophore is consistent with orientation of the SB proton toward the cytoplasmic surface in the N intermediate.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 009609264

Download citation: RISBibTeXText

Related references

Solid state carbon 13 nmr of the m 412 photointermediate of bacteriorhodopsin. Biophysical Journal 53(2 Part 2): 443A, 1988

Chromophore structure in bacteriorhodopsin's O640 photointermediate. Biochemistry 22(26): 6141-6148, 1983

Insight into the chromophore of rhodopsin and its Meta-II photointermediate by 19 F solid-state NMR and chemical shift tensor calculations. Physical Chemistry Chemical Physics, PCCP 20(48): 30174-30188, 2018

Solid state carbon-13 NMR rotational resonance distance measurement in the M photointermediate of bacteriorhodopsin Determination of the configuration about the Schiff base. Biophysical Journal 64(2 Part 2): A212, 1993

Internuclear distance measurement in a reaction intermediate Solid-state carbon-13 NMR rotational resonance determination of the Schiff base configuration in the M photointermediate of bacteriorhodopsin. Journal of the American Chemical Society 115(18): 8515-8516, 1993

Chromophore motion during the bacteriorhodopsin photocycle: polarized absorption spectroscopy of bacteriorhodopsin and its M-state in bacteriorhodopsin crystals. Embo Journal 10(9): 2353-2361, 1991

Solid-state 13C NMR detection of a perturbed 6-s-trans chromophore in bacteriorhodopsin. Biochemistry 24(24): 6955-6962, 1985

Solid-state 13C NMR of the retinal chromophore in photointermediates of bacteriorhodopsin: characterization of two forms of M. Biochemistry 28(1): 237-243, 1989

Chromophore distortions in the bacteriorhodopsin photocycle: evolution of the H-C14-C15-H dihedral angle measured by solid-state NMR. Biochemistry 41(2): 431-438, 2002

Solid state carbon 13 nmr and nitrogen 15 nmr investigations of the retinal protonated schiff base chromophore in bacteriorhodopsin. Chien, S And C Ho (Ed ) Nmr in Biology And Medicine; Symposium, Taipei, Taiwan, Dec 1984 Xviii+248p Raven Press: New York, N Y , Usa Illus 33-44, 1986

Structure and protein environment of the retinal chromophore in light- and dark-adapted bacteriorhodopsin studied by solid-state NMR. Biochemistry 28(22): 8897-8904, 1989

Generation of the O630 photointermediate of bacteriorhodopsin is controlled by the state of protonation of several protein residues. Biochemistry 38(7): 2018-2025, 1999

The angles between the C(1)-, C(5)-, and C(9)-methyl bonds of the retinylidene chromophore and the membrane normal increase in the M intermediate of bacteriorhodopsin: direct determination with solid-state (2)H NMR. Biochemistry 38(36): 11762-11772, 1999

Deuterium solid-state nuclear magnetic resonance studies of methyl group dynamics in bacteriorhodopsin and retinal model compounds: Evidence for a 6-s-trans chromophore in the protein. Biochemistry 33(11): 3280-3286, 1994

Solid-state 13C-NMR of [(3-13C)Pro]bacteriorhodopsin and [(4-13C)Pro]bacteriorhodopsin: evidence for a flexible segment of the C-terminal tail. European Journal of Biochemistry 235(3): 526-533, 1996