+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

The receptor for mouse hepatitis virus in the resistant mouse strain SJL is functional: implications for the requirement of a second factor for viral infection



The receptor for mouse hepatitis virus in the resistant mouse strain SJL is functional: implications for the requirement of a second factor for viral infection



Journal of Virology 66(12): 6931-6938



The SJL mouse strain is resistant to infection by some strains of the murine coronavirus mouse hepatitis virus (MHV), such as JHM and A59. The block to virus infection has been variously attributed to defects in virus receptors or virus spread. Since the cellular receptors for MHV, mmCGM1 and mmCGM2, have recently been identified as members of the carcinoembryonic antigen family, we reexamined the possible defectiveness of the MHV receptors in SJL mouse strain. Cloning and sequencing of the cDNAs of both mmCGMs RNAs from SJL mice revealed that they were identical in size to those of the susceptible C57BL/6 (B6) mouse. There was some sequence divergence in the N terminus of the mmCGM molecules between the two mouse strains, resulting in a different number of potential glycosylation sites. This was confirmed by in vitro translation of the mmCGM RNAs, which showed that the glycosylated mmCGM2 of SJL was smaller than that of B6 mice. However, transfection of either mmCGM1 or MMCGM2 from SJL mice into MHV-resistant Cos 7 cells rendered the cells susceptible to MHV infection. The ability of the SJL mmCGM molecules to serve as MHV receptors was comparable to that of those from B6. These molecules are expressed in SJL mouse brain and liver in a similar ratio and in amounts equivalent to those in the B6 mouse. Furthermore, we demonstrated that an SJL-derived cell line was susceptible to A59 but resistant to JHM infection. We concluded that the MHV receptor molecules in the SJL mouse are functional and that the resistance of SJL mice to infection by some MHV strains most likely results from some other factor(s) required for virus entry or some other step(s) in virus replication.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 009613392

Download citation: RISBibTeXText

PMID: 1279194


Related references

Differential growth of mouse hepatitis virus strain pri and mouse hepatitis virus strain c 3h in genetically resistant c 3h mice rendered susceptible by eperythrozoon infection. Archiv fuer die Gesamte Virusforschung 41(3): 175-184, 1973

Mouse susceptibility to mouse hepatitis virus infection is linked to viral receptor genotype. Journal of Virology 71(11): 8860-8863, 1997

Responses of mice susceptible or resistant to lethal infection with mouse hepatitis virus, strain JHM, after exposure by a natural route. Laboratory Animal Science 35(5): 469-472, 1985

In vitro splenic t cell responses of diverse mouse genotypes after infection with mouse hepatitis virus strain jhm. Laboratory Animal Science 40(5): 543, 1990

A spike protein-dependent cellular factor other than the viral receptor is required for mouse hepatitis virus entry. Virology 196(1): 45-56, 1993

Induction of natural killer cells and interferon during mouse hepatitis virus infection of resistant and susceptible inbred mouse strains. Immunobiology 166(1): 35-44, 1984

Down-regulation of Bgp1a viral receptor by interferon-c is related to the antiviral state and resistance to mouse hepatitis virus 3 infection. Virology 274(2): 8-83, 2000

Purification of the 110-kilodalton glycoprotein receptor for mouse hepatitis virus (MHV)-A59 from mouse liver and identification of a nonfunctional, homologous protein in MHV-resistant SJL/J mice. Journal of Virology 64(8): 3817-3823, 1990

Mouse Hepatitis Virus Receptor as a Determinant of the Mouse Susceptibility to MHV Infection. Frontiers in Microbiology 3: 68, 2012

Interleukin 6 induction by mouse hepatitis virus mhv 4 jhm infection of mouse brain endothelial cells and astrocytes derived from susceptible and resistant strains of mice. FASEB Journal 5(6): A1780, 1991

Down-regulation of Bgp1(a) viral receptor by interferon-gamma is related to the antiviral state and resistance to mouse hepatitis virus 3 infection. Virology 274(2): 278-283, 2000

Mouse hepatitis virus infection utilizes more than one receptor and requires an additional cellular factor. Advances in Experimental Medicine and Biology 342: 273-277, 1993

Transfer of the gene for susceptibility to mouse hepatitis virus into a genetically resistant strain of mice further data establishing the role of macrophages in mouse susceptibility. Bacteriological Proceedings 71: 202, 1971

Mouse hepatitis virus strain 3 infection of C57, A/Sn and A/J strain mice and their macrophages. Brief report. Archives of Virology 66(1): 71-75, 1980

Generation of congenic mouse strains by introducing the virus-resistant genes, Mx1 and Oas1b, of feral mouse-derived inbred strain MSM/Ms into the common strain C57BL/6J. Japanese Journal of Veterinary Research 57(2): 89-99, 2009