EurekaMag.com logo
+ Site Statistics
References:
53,517,315
Abstracts:
29,339,501
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

The requirement for Vif of SIVmac is cell-type dependent



The requirement for Vif of SIVmac is cell-type dependent



Journal of General Virology 77: 427-434



The vif gene (viral infectivity factor) of the human and simian immunodeficiency viruses (HIV and SIV) is present in almost all members of the lentivirus group of retroviruses. This gene is highly conserved among different HIV and SIV isolates and is therefore presumed to play an important role in pathogenesis. To analyse the role of Vif in SIV, three SIVmac mutants have been constructed by introducing site-specific mutations or deletions into vif of the pathogenic molecular clone SIVmac239. The effect of Vif on viral replication in T cells was examined by transfecting equal amounts of either vif-positive or vif-negative viral DNA into SupT1, CEM-SS and H9 cells. Reverse transcriptase assay of supernatants from transfected cultures revealed that both SupT1 and CEM-SS cell lines supported replication of all three vif mutants to a level comparable to the parental vif-positive virus, whereas vif mutants did not replicate in H9 cells. Our results demonstrate that the requirement for Vif in SIVmac replication is cell-type dependent and that sequences near both the N and C termini are required for its function. Vif-defective SIVmac239, produced in transfected SupT1 and CEM-SS cells, failed to infect primary T lymphocytes, whereas both vif-positive and vif-defective viruses established productive infection in CEMx174 cells. These findings in primary cells suggest that Vif plays an important role in viral replication in vivo.

(PDF same-day service: $19.90)

Accession: 009616885

Download citation: RISBibTeXText

PMID: 8601777

DOI: 10.1099/0022-1317-77-3-427



Related references

Cell type-dependent requirement of autophagy in HSV-1 antiviral defense. Autophagy 9(2): 236-238, 2013

Cell type-dependent requirement for PIP box-regulated Cdt1 destruction during S phase. Molecular Biology of the Cell 21(21): 3639-3653, 2011

The translational requirement for complete la crosse virus messenger rna synthesis is cell type dependent. Journal of Virology 63(12): 5159-5165, 1989

The translational requirement for complete La Crosse virus mRNA synthesis is cell-type dependent. Journal of Virology 63(12): 5159-5165, 1989

Cell-dependent requirement of human immunodeficiency virus type 1 gp41 cytoplasmic tail for Env incorporation into virions. Journal of Virology 74(10): 4891-4893, 2000

Cell-dependent requirement of human immunodeficiency virus type 1 Vif protein for maturation of virus particles. Journal of Virology 67(3): 1663-1666, 1993

Presence of circulating CTL against SIV ENV, RT or NEF induced by SIVMAC J5 or SIVMAC C8 does not correlate with protection against SIHV896P challenge. Journal of Medical Primatology 27(2-3): 150-151, April-June, 1998

Failure of SIVmac to be neutralized in macrophage cultures is unique to SIVmac and not observed with neutralization of SHIV or HIV-1. Aids Research and Human Retroviruses 14(12): 1045-1051, 1998

Enhanced T- and B-cell responses to simian immunodeficiency virus (SIV)agm, SIVmac and human immunodeficiency virus type 1 Gag DNA immunization and identification of novel T-cell epitopes in mice via codon optimization. Journal of General Virology 90(Pt 10): 2513-2518, 2009

Cross-reactivity with SIVmac in east African HIV-1-positive sera: evidence against double infection with HIV-1 and a SIVmac/HIV-2-like virus. Intervirology 31(5): 276-284, 1990

Antibody-dependent cellular cytotoxicity detects type- and strain-specific antigens among human immunodeficiency virus types 1 and 2 and simian immunodeficiency virus SIVmac isolates. Journal of Virology 63(8): 3376-3381, 1989

Replication of human immunodeficiency virus type 1 (HIV-1), simian immunodeficiency virus strain mac (SIVmac) and chimeric HIV-1/SIVmac viruses having env genes derived from macrophage-tropic viruses: an indication of different mechanisms of macrophage-tropism in human and monkey cells. Journal of General Virology 79: 741-745, 1998

A single vaccination with attenuated SIVmac 239 via the tonsillar route confers partial protection against challenge with SIVmac 251 at a distant mucosal site, the rectum. Frontiers in Bioscience 12: 2107-2123, 2006

A single vaccination with attenuated SIVmac 239 via the tonsillar route confers partial protection against challenge with SIVmac 251 at a distant mucosal site, the rectum. Frontiers in Bioscience 12: 2107-2123, 2007