EurekaMag
+ Most Popular
Cunninghamia lanceolata plantations in China
Mammalian lairs in paleo ecological studies and palynology
Studies on technological possibilities in utilization of anhydrous milk fat for production of recombined butter-like products
Should right-sided fibroelastomas be operated upon?
Large esophageal lipoma
Apoptosis in the mammalian thymus during normal histogenesis and under various in vitro and in vivo experimental conditions
Poissons characoides nouveaux ou non signales de l'Ilha do Bananal, Bresil
Desensitizing efficacy of Colgate Sensitive Maximum Strength and Fresh Mint Sensodyne dentifrices
Administration of fluid by subcutaneous infusion: revival of a forgotten method
Tundra mosquito control - an impossible dream?
Schizophrenia for primary care providers: how to contribute to the care of a vulnerable patient population
Geochemical pattern analysis; method of describing the Southeastern limestone regional aquifer system
Incidence of low birth weights in a hospital of Mexico City
Tabanidae
Graded management intensity of grassland systems for enhancing floristic diversity
Microbiology and biochemistry of cheese and fermented milk
The ember tetra: a new pygmy characid tetra from the Rio das Mortes, Brazil, Hyphessobrycon amandae sp. n. (Pisces, Characoidei)
Risk factors of contrast-induced nephropathy in patients after coronary artery intervention
Renovation of onsite domestic wastewater in a poorly drained soil
Observations of the propagation velocity and formation mechanism of burst fractures caused by gunshot
Systolic blood pressure in a population of infants in the first year of life: the Brompton study
Haematological studies in rats fed with metanil yellow
Studies on pasteurellosis. I. A new species of Pasteurella encountered in chronic fowl cholera
Dormancy breaking and germination of Acacia salicina Lindl. seeds
therapy of lupus nephritis. a two-year prospective study

The role of Hsp70 in conferring unidirectionality on protein translocation into mitochondria


The role of Hsp70 in conferring unidirectionality on protein translocation into mitochondria



Science 266(5188): 1250-1253



ISSN/ISBN: 0036-8075

PMID: 7973708

DOI: 10.1126/science.7973708

The entry of segments of preproteins of defined lengths into the matrix space of mitochondria was studied. The mitochondrial chaperone Hsp70 (mtHsp70) interacted with proteins emerging from the protein import channel and stabilized translocation intermediates across the membranes in an adenosine triphosphate-dependent fashion. The chaperone bound to the presequence and mature parts of preproteins. In the absence of mtHsp 70 binding, preproteins with less than 30 to 40 residues in the matrix diffused out of mitochondria. Thus, protein translocation was reversible up to a late stage. The import channels in both mitochondrial membranes constitute a passive pore that interacts weakly with polypeptide chains entering the matrix.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 009618428

Download citation: RISBibTeXText

Related references

Role of protein lipid interactions in the mechanism of protein translocation into mitochondria. Biophysical Journal 61(2 Part 2): A86, 1992

Protein translocation into mitochondria: the role of TIM complexes. Trends in Cell Biology 10(1): 25-31, 2000

On the possible role of structural protein in the binding and translocation of adenine nucleotides in mitochondria. Biochimica et Biophysica Acta 131(3): 582-585, 1967

Contact sites between inner and outer membranes: structure and role in protein translocation into the mitochondria. Biochimica et Biophysica Acta 1018(2-3): 239-242, 1990

Role of JNK translocation to mitochondria leading to inhibition of mitochondria bioenergetics in acetaminophen-induced liver injury. Journal of Biological Chemistry 283(20): 13565-13577, 2008

Role of Jnk Translocation to Mitochondria Leading to Inhibition of Mitochondria Bioenergetics in Acetaminophen-Induced Liver Injury. Gastroenterology 134(4): A-766, 2008

A mitochondrial membrane protein is required for translocation of phosphatidylserine from mitochondria-associated membranes to mitochondria. Biochemical Journal 331: 217-223, 1998

A mitochondrial surface protein mediates translocation of phosphatidylserine from endoplasmic reticulum-like, mitochondria-associated membranes to mitochondria. Molecular Biology of the Cell 7(Suppl ): 138A, 1996

P53 translocation to mitochondria precedes its nuclear translocation and targets mitochondrial oxidative defense protein-manganese superoxide dismutase. Cancer Research 65(9): 3745-3750, 2005

Translocation arrest by reversible folding of a precursor protein imported into mitochondria. A means to quantitate translocation contact sites. Journal of Cell Biology 109(4 Pt 1): 1421-1428, 1989

Molecular cloning and sequence of cDNAs for the import precursors of oligomycin sensitivity conferring protein, ATPase inhibitor protein, and subunit c of H(+)-ATP synthase in rat mitochondria. Biochimica et Biophysica Acta 1172(3): 311-314, 1993

The oligomycin sensitivity conferring protein of beef heart mitochondria studies of its binding to f 1 and its function. Journal of Bioenergetics and Biomembranes 16(5-6): 535-550, 1984

Stabilization and translocation of p53 to mitochondria is linked to Bax translocation to mitochondria in simvastatin-induced apoptosis. Biochemical and Biophysical Research Communications 391(4): 1592-1597, 2010

The oligomycin sensitivity conferring protein (OSCP) of beef heart mitochondria: studies of its binding to F1 and its function. Journal of Bioenergetics and Biomembranes 16(5-6): 535-550, 1984

ATP synthase complex from bovine heart mitochondria. Passive H+ conduction through F0 does not require oligomycin sensitivity-conferring protein. Journal of Biological Chemistry 265(13): 7632-7637, 1990