The role of epidermis and lipids of stratum corneum in skin barrier function of guinea pig

Lee, J.Y.; Houh, W.

Journal of Catholic Medical College 45(4): 1417-1424


Accession: 009620030

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Stratum corneum plays a role of protective barrier and its protective function mainly depends on the lipids of stratum corneurm. Many kinds of skin disease develop when toxic materials are impacted on the skin of which barrier function is already disturbed by various insults. In order to investigate the role of epidermis and lipids of stratum corneum in skin barrier function of guinea pigs, percutaneous absorption and lipid composition of stratum corneum were evaluated in the white guinea pigs skin of various conditions including normal (group A), removal of skin (group B), removal of stratum corneum (group C), single application of solvent (Group D), eight consecutive applications of solvent (group E) and correction ring of percutaneous absorption (group F). Percutaneous absorption was determined by indirect method of measuring the radioactivity in urine for 5 days following topical application of C-14-hydrocortisone in group A, B, C, D, and E. Lipids of stratum corneum were evaluated by high performance thin layer chromatography and laser densitometer in group A, D and E. The results were as follows: Patterns of percutaneous absorption of C-14-hydrocortisone in each group for 5 days were different (P lt 0.05). Especially on day 1, absorbed doses (%) of C-14-hydrocortisone of group B(34.1 +- 14.0) and group C(25.1 +- 15.2) were increased statistically than the absorbed doses of group A(0.9 +- 0.2), D(0.8 +- 0.2) and E(1.0 +- 0.4). and E(1.0 +- 0.4) (P lt 0.05). Absorbed doses of each group were not different on day 2 to day 5. The major group of lipid in stratum corneum of normal guinea pig was the sphingolipids, which comprise 37.7 +- 2.5% of the total lipid and then free sterol compromise 22.8 +- 3.0%, sterol ester 12.6% +- 1.1%, free fatty acids 9.7 +- 2.2%, triglyceride 8.5 +- 1.1%, phospholipids 6.8 +- 1.7% and cholesterol sulfate comprise 2.1 +- 2.6% of total lipid. Lipid composition of group D was not different from group A, but lipid composition of group E was different from group A and D. Sphingolipids of group E(72.3 +- 28.0), expressed as aribitrary unit, increased statistically than sphingolipids of group A(25.8 +- 4.2) and D(21.8 +- 6.7) (P lt 0.05), but other lipid components of group E were not different from those of group A and D.