+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

The sequence and structure of the 3'-untranslated regions of chloroplast transcripts are important determinants of mRNA accumulation and stability



The sequence and structure of the 3'-untranslated regions of chloroplast transcripts are important determinants of mRNA accumulation and stability



Plant Molecular Biology 36(2): 307-314



A general characteristic of the 3'-untranslated regions (3' UTRs) of plastid mRNAs is an inverted repeat (IR) sequence that can fold into a stem-loop structure. These stem-loops are RNA 3'-end processing signals and determinants of mRNA stability, not transcription terminators. Incubation of synthetic RNAs corresponding to the 3' UTRs of Chlamydomonas chloroplast genes atpB and petD with a chloroplast protein extract resulted in the accumulation of stable processing products. Synthetic RNAs of the petA 3' UTR and the antisense strand of atpB 3' UTR were degraded in the extract. To examine 3' UTR function in vivo, the atpB 3' UTR was replaced with the 3' UTR sequences of the Chlamydomonas chloroplast genes petD, petD plus trnR plus trnR, rbcL, petA and E. coli thrA by biolistic transformation of Chlamydomonas chloroplasts. Each 3' UTR was inserted in both the sense and antisense orientations. The accumulation of both total atpB mRNA and ATPase beta-subunit protein in all transformants was increased compared to a strain in which the atpB 3' UTR had been deleted. However, the level of discrete atpB transcripts in transformants containing the antisense 3' UTR sequences was reduced to approximately one-half that of transformants containing the 3' UTRs in the sense orientation. These results imply that both the nucleotide sequences and the stem-loop structures of the 3' UTRs are important for transcript 3'-end processing, and for accumulation of the mature mRNAs.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 009625000

Download citation: RISBibTeXText

PMID: 9484442

DOI: 10.1023/a:1005943701253


Related references

RNA stability and translational regulatory elements in the 5' untranslated regions of two Chlamydomonas chloroplast transcripts. Nucleic Acids Symposium Series 36: 132-134, 1997

The effect of different 3' untranslated regions on the accumulation and stability of transcripts of a gfp transgene in chloroplasts of transplastomic tobacco. Plant Molecular Biology 76(3-5): 385-396, 2011

Interactions of chloroplast mRNA 3 untranslated regions with protein that promote RNA processing and stability. Journal of Cellular Biochemistry Suppl. 0(18C): 149, 1994

Specific sequence elements in the 5' untranslated regions of rbcL and atpB gene mRNas stabilize transcripts in the chloroplast of Chlamydomonas reinhardtii. Rna 7(7): 1024-1033, 2001

Mutations that alter the higher-order structure of its 5' untranslated region affect the stability of chloroplast rps7 mRNA. Molecular and General Genetics 264(3): 291-299, 2000

Distinct roles for the 5' and 3' untranslated regions in the degradation and accumulation of chloroplast tufA mRNA: identification of an early intermediate in the in vivo degradation pathway. Plant Molecular Biology 63(5): 689-702, 2007

Structure and expression of the guinea pig preproenkephalin gene: site-specific cleavage in the 3' untranslated region yields truncated mRNA transcripts in specific brain regions. Molecular and Cellular Biology 15(4): 2080-2089, 1995

Bovine chondrocyte link protein cDNA sequence: interspecies conservation of primary structure and mRNA untranslated regions. Comparative Biochemistry and Physiology B, Biochemistry and Molecular Biology 112(2): 197-203, 1995

The 3 untranslated regions of the two alternative type XVII collagen mRNA transcripts have different effects on translation efficiency. Journal of Investigative Dermatology 123(2), 2004

Cytidine-rich protein-binding sequence in the 3 untranslated region of tyrosine hydroxylase mRNA as a determinant of the TH mRNA stability. Society for Neuroscience Abstracts 22(1-3): 1559, 1996

5' sequences are important positive and negative determinants of the longevity of Chlamydomonas chloroplast gene transcripts. Proceedings of the National Academy of Sciences of the United States of America 90(4): 1556-1560, 1993

The turnip yellow mosaic virus tRNA-like structure cannot be replaced by generic tRNA-like elements or by heterologous 3' untranslated regions known to enhance mRNA expression and stability. Journal of Virology 70(4): 2107-2115, 1996