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The sequence divergence in NS3 region of HGV/GBV-C



The sequence divergence in NS3 region of HGV/GBV-C



Zhonghua Weishengwuxue He Mianyixue Zazhi 17(2): 96-98



The new Hepatitis virus (HGV/GBV-C) was detected in sera from nonA-E hepatitis with using RT-PCR. The reaction for amplifying NS3 region of HGV/GBV-C was a single round touchdown PCR of 53 cycles with degenerate PCR primers. The positive products were cloned and sequenced. Nucleotide identity in NS3 between HGV and samples was between 82.0% apprx 91.4% and that between GBV-C and samples was between 78.4% apprx 84.2%. So the sequence divergence of new hepatitis virus (HGV/ GBV-C) may be high.

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