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The somatic cell-specific low density lipoprotein receptor-related protein of the chicken. Close kinship to mammalian low density lipoprotein receptor gene family members



The somatic cell-specific low density lipoprotein receptor-related protein of the chicken. Close kinship to mammalian low density lipoprotein receptor gene family members



Journal of Biological Chemistry 269(1): 212-219



Recently, the family of mammalian genes homologous to that for the low density lipoprotein (LDL) receptor has grown. One of the new family members, termed LDL receptor-related protein/alpha-2-macroglobulin receptor (LRP/alpha-2MR), is one of the largest cell surface proteins characterized to date. Its functions have been hypothesized to include the plasma clearance of chylomicron remnants and activated alpha-2-macroglobulin, as well as the local metabolism of complexes between plasminogen activators and their endogenous inhibitors. Here we describe the molecular characterization of an LRP/alpha-2MR expressed in chickens, which do not metabolize chylomicron remnants. This chicken protein is expressed in somatic tissues and is different from a second LRP/alpha-2MR exclusively expressed in growing ovarian follicles. The sequence of the somatic cell-specific chicken LRP/alpha-2MR, deduced from cloned full-length cDNA, shows 83% overall identity with human LRP/alpha-2MR. Important characteristic features of the modular protein are completely conserved; in particular, all cysteine residues align perfectly. The avian LRP/alpha-2MR is post-translationally cleaved in the same fashion as its human counterpart, and the resulting 515-kDa extracellular subunit binds Ca-2+, alpha-2-macroglobulin, and vitellogenin. The results indicate that avian LRP/alpha-2MR genes have emerged from an ancestor designed to ensure a pivotal event in the reproduction of oviparous species, i.e. vitellogenesis, and that mammalian LRP/alpha-2MRs have acquired features required for functioning in plasma clearance of certain non-yolk proteins.

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Accession: 009626725

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PMID: 7506255


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