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The sugar beet mitochondrial gene for the ATPase alpha-stimuli: Sequence, transcription and rearrangements in cytoplasmic male-sterile plants

The sugar beet mitochondrial gene for the ATPase alpha-stimuli: Sequence, transcription and rearrangements in cytoplasmic male-sterile plants

Current Genetics 24(1-2): 164-170

We have characterized the mitochondrial atpA (the alpha subunit of F-1-ATPase) gene from male-fertile cytoplasm (cv TK81-0) of sugar beet. The gene is 1518-bp long and encodes a polypeptide of 506 amino acids. The atpA mRNA sequence is modified by three C-to-U RNA editing events, all of which alter the encoded protein sequences. In order to examine the genome organization of the atpA locus in cytoplasmic male-sterile (CMS) sugar beet, atpA-containing clones were isolated from Owen CMS (TK81-MS) and a different source of CMS (I12CMS(2)) cytoplasm respectively. The sequences of the atpA coding region from TK81-MS and I-12CMS(2) are identical to each other and to the corresponding TK81-0 sequence. However, the TK81-0 and TK81-MS loci diverge completely 47 bp upstream of the initiation codon. resulting in different 5' transcript termini for the two genes. On the other hand, the point of divergence between theTK81-0 and 1-12CMS(2) atpA genes was found to occur after 393 bp 3' to the TAA stop codon. Our results also show the 3'-flanking sequences of I-12CMS(2) atpA to be present elsewhere in the mitochondrial genomes of TK81-0, TK81-MS and I12CMS(2), suggesting the possible involvement of these repeated DNA elements in the sequence rearrangements.

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