+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Tissue-specific expression of two alternatively spliced isoforms of the human insulin receptor protein



Tissue-specific expression of two alternatively spliced isoforms of the human insulin receptor protein



Acta Diabetologica 31(2): 59-65



Two insulin receptor mRNA species are expressed in human tissues as a result of alternative splicing of exon 11. This event is regulated in a tissue-specific manner. To date, there is little information about the relative abundance of the two receptor protein isoforms on the cell surface. The aim of the present investigation was to assess whether the tissue-specific expression of the two insulin receptor mRNA species is paralleled by a similar pattern of expression of the two receptor protein isoforms. To this end, we assessed the relative distribution of the two receptor variants in various human tissues at the mRNA and protein levels. A PCR-based technique was used to measure the relative abundance of the two mRNA species, and two immunological assays were used to measure the relative steady-state expression of the two receptor protein isoforms. The expression of the two insulin receptor protein isoforms followed the tissue-specific pattern of expression of the two mRNA species.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 009652494

Download citation: RISBibTeXText

PMID: 7949223

DOI: 10.1007/bf00570536


Related references

Tissue-specific expression of two alternatively spliced insulin receptor mRNAs in man. Molecular Endocrinology 3(8): 1263-1269, 1989

Tissue specific expression of alternatively spliced murine PECAM-1 isoforms. Developmental Dynamics 214(1): 44-54, 1999

Binding of human, porcine and bovine insulin to insulin receptors from human brain, muscle and adipocytes and to expressed recombinant alternatively spliced insulin receptor isoforms. Diabetologia 38(7): 757-763, 1995

Tissue specific expression and function of alternatively spliced murine PECAM-1 isoforms. Molecular Biology of the Cell 8(Suppl. ): 284A, 1997

The tissue specific distribution of alternatively spliced human PECAM-1 isoforms. Molecular Biology of the Cell 12(Suppl.): 480a, 2001

Tissue-specific distributions of alternatively spliced human PECAM-1 isoforms. American Journal of Physiology. Heart and Circulatory Physiology 284(3): H1008, 2003

Alternatively spliced variants of the insulin receptor protein. Expression in normal and diabetic human tissues. Journal of Clinical Investigation 89(6): 2066-2070, 1992

Two discrete promoters regulate the alternatively spliced human interferon regulatory factor-5 isoforms. Multiple isoforms with distinct cell type-specific expression, localization, regulation, and function. Journal of Biological Chemistry 280(22): 21078-21090, 2005

A new methodology for quantification of alternatively spliced exons reveals a highly tissue-specific expression pattern of WNK1 isoforms. Plos one 7(5): E37751, 2012

Expression of alternatively spliced growth factor receptor isoforms in the human trabecular meshwork. Investigative Ophthalmology and Visual Science 40(1): 242-247, 1999

Novel alternatively spliced isoforms of the neurofibromatosis 2 tumor suppressor demonstrate tissue-specific expression pattern and distinct subcellular localization. American Journal of Human Genetics 65(4): A321, 1999

Differential expression of alternatively spliced 5-HT-7 serotonin receptor isoforms in human brain versus smooth muscle. Society for Neuroscience Abstracts 23(1-2): 127, 1997

Three different turkey luteinizing hormone receptor (tLH-R) isoforms I: characterization of alternatively spliced tLH-R isoforms and their regulated expression in diverse tissues. Biology of Reproduction 62(1): 108-116, 2000

Three Different Turkey Luteinizing Hormone Receptor (tLh-R) Isoforms I: Characterization of Alternatively Spliced tLh-R Isoforms and Their Regulated Expression in Diverse Tissues. Biology of Reproduction 62(1): 108-116, 2000

Tissue-specific expression of alternatively spliced forms of integrin associated protein. Molecular Biology of the Cell 5(Suppl. ): 428A, 1994