+ Translate
+ Most Popular
The pigeon tick (Argas reflexus): its biology, ecology, and epidemiological aspects
Prevalence of hemoglobin abnormalities in Kebili (Tunisian South)
Lipogranuloma: a preventable complication of dacryocystorhinostomy
Value of basal plasma cortisol assays in the assessment of pituitary-adrenal insufficiency
Bees from the Belgian Congo. The acraensis group of Anthophora
Placing gingival retraction cord
Total serum IgE, allergy skin testing, and the radioallergosorbent test for the diagnosis of allergy in asthmatic children
Acariens plumicoles Analgesoidea parasites des oiseaux du Maroc
Injuries of terminal phalanges of the fingers in children
Biology of flowering and nectar production in pear (Pyrus communis)
Das Reliktvorkommen der Aspisviper (Vipera aspis L.) im Schwarzwald
Hydrological modelling of drained blanket peatland
Pathologic morphology and clinical significance of the anomalous origin of the left circumflex coronary artery from the right coronary artery. General review and autopsy analysis of 30 cases
Cyto genetic analyses of lymphocyte cultures after exposure to calcium cyclamate
Axelrodia riesei, a new characoid fish from Upper Rio Meta in Colombia With remarks concerning the genus Axelrodia and description of a similar, sympatric, Hyphessobrycon-species
Favorable evolution of a case of tuberculosis of pancreas under antibiotic action
RIFM fragrance ingredient safety assessment, Valencene, CAS Registry Number 4630-07-3
Parenteral microemulsions: an overview
Temperate pasture: management for grazing and conservation
Evaluation of a new coprocessed compound based on lactose and maize starch for tablet formulation
Thermal expansion and cracking of three confined water-saturated igneous rocks to 800C
Revision of the genera of the tribe Stigmoderini (Coleoptera: Buprestidae) a discussion of phylogenetic relationships
Anal tuberculosis. Report of a case
Gastric tuberculosis in the past and present
Adaptive responses of the cardiovascular system to prolonged spaceflight conditions: assessment with Holter monitoring

Total synthesis and expression in Escherichia coli of a gene encoding human tropoelastin

Total synthesis and expression in Escherichia coli of a gene encoding human tropoelastin

Gene 154(2): 159-166

ISSN/ISBN: 0378-1119

PMID: 7890158

DOI: 10.1016/0378-1119(94)00848-m

To elucidate the structural features and interactions of tropoelastin (TEL) molecules which assist in giving the elastic fibre its physical properties, a 2210-bp synthetic human TEL-encoding gene (SHEL) was constructed for expression in Escherichia coli. To this end, a model of codon adjustment was tested which better suits the polypeptide biosynthetic needs of E. coli than the human sequence, where over one-third of this natural sequence contains expression-limiting rare codons and 4 amino acids alone account for 75% of the resulting polypeptide. This large synthetic TEL gene was expressed at a high level as the recombinant counterpart of human TEL and as a C-terminal fusion with glutathione S-transferase. This demonstrates that a synthetic approach based upon matching codon usage to that of the host organism can support significant expression of recombinant sequences. The synthetic gene incorporates the facility for simple cassette replacement in future insertion, deletion and mutagenesis experiments, including the introduction and removal of exon homologues. The resulting soluble polypeptide is easily purified and displays properties expected for this protein.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 009656081

Download citation: RISBibTeXText

Related references

Chemical synthesis of a gene encoding the human complement fragment C5a and its expression in Escherichia coli. Proceedings of the National Academy of Sciences of the United States of America 82(11): 3543-3547, 1985

Synthesis of a gene encoding bovine substance P precursors and its expression in Escherichia coli. Gene 117(2): 259-263, 1992

Expression of the human gene encoding cytochrome p 450 2e1 in escherichia coli. Biophysical Journal 61(2 Part 2): A322, 1992

Increased expression in Escherichia coli of a synthetic gene encoding human somatomedin C after gene duplication and fusion. Journal of Bacteriology 169(12): 5385-5392, 1987

Expression of a synthetic gene encoding human transthyretin in Escherichia coli. Protein Expression and Purification 30(1): 55-61, 2003

Inactivation of the Escherichia coli B41 (O101:K99/F41) rfb gene encoding an 80-kDa polypeptide results in the synthesis of an antigenically altered lipopolysaccharide Escherichia coli K-12. Gene (Amsterdam) 123(1): 9-15, 1993

Expression of a gene, encoding a soluble part of CD4 receptor of human T-lymphocytes in Escherichia coli. Biotekhnologiya 1995(5-6): 11-15, 1995

High-level expression of a gene encoding the human complement factor C5a in Escherichia coli. Gene 43(1-2): 131-138, 1986

The expression of the gene encoding human granulocyte colony stimulating factor in Escherichia coli. Biotekhnologiya 1998(6): 24-35, 1998

Total synthesis of the gene coding for mammalian cytochrome b 5 and its expression in escherichia coli. Federation Proceedings 45(6): 1855, 1986

Physical characterisation of the Escherichia coli B gene encoding nitroreductase and its over-expression in Escherichia coli K12. Fems Microbiology Letters 124(2): 195-202, 1994

Fusion and expression of the gene encoding human Mn-SOD to anti-CEA single-chain antibody in Escherichia coli. Sheng Wu Gong Cheng Xue Bao 16(5): 566-569, 2000

Total chemical synthesis and expression in Escherichia coli of a maize glutathione-transferase (GST) gene. Gene 76(1): 153-160, 1989

Total synthesis and expression in escherichia coli of human interleukin 2 complementary dna. Protein Engineering 3(4): 382, 1990

Structural organization of the gene encoding the human lipocalin tear prealbumin and synthesis of the recombinant protein in Escherichia coli. Gene 139(2): 177-183, 1994