+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Transcription termination in the Escherichia coli dnaA gene is not mediated by the internal DnaA box

Transcription termination in the Escherichia coli dnaA gene is not mediated by the internal DnaA box

Journal of Bacteriology 177(7): 1896-1899

DnaA protein is a DNA-binding protein which recognizes a 9-bp consensus sequence called the DnaA box. By binding to DnaA boxes, DnaA protein regulates initiation of chromosomal replication and transcription of several genes. The dnaA gene contains two DnaA boxes, one located in the regulatory region and one within the structural gene. In this paper, we explore the role of the internal DnaA box in dnaA expression because it has been proposed that the DnaA box-DnaA protein complex can block transcribing RNA polymerase. Firstly, we analyzed the degree of derepression of the dnaA gene, measured as beta-galactosidase activity of a dnaA-lacZ fusion inserted onto the bacterial chromosome, produced by an extra copy number of the dnaA DnaA boxes carried by multicopy plasmids. Secondly, we analyzed repression produced by elevated levels of DnaA protein on single-copy dnaA-lacZ fusions containing or not containing the internal DnaA box. Our results indicate that the internal DnaA box does not play a regulatory role in dnaA expression.

Please choose payment method:

(PDF emailed within 1 workday: $29.90)

Accession: 009661312

Download citation: RISBibTeXText

PMID: 7896719

Related references

Titration of DnaA protein by oriC DnaA-boxes increases dnaA gene expression in Escherichia coli. Embo Journal 6(1): 255-258, 1987

DnaA and LexA Proteins Regulate Transcription of the uvrB Gene in Escherichia coli : The Role of DnaA in the Control of the SOS Regulon. Frontiers in Microbiology 9: 1212, 2018

Hda-mediated inactivation of the DnaA protein and dnaA gene autoregulation act in concert to ensure homeostatic maintenance of the Escherichia coli chromosome. Genes and Development 20(15): 2121-2134, 2006

Termination of the Escherichia coli asnC transcript. The DnaA protein/dnaA box complex blocks transcribing RNA polymerase. Gene 73(2): 347-354, 1988

The presence of two DnaA-binding sequences is required for an efficient interaction of the Escherichia coli DnaA protein with each particular weak DnaA box region. FEMS Microbiology Letters 174(1): 25-31, 1999

Multiple pathways regulating DnaA function in Escherichia coli: Distinct roles for DnaA titration by the datA locus and the regulatory inactivation of DnaA. Biochimie 83(1): 13-17, 2001

Directionality of DnaA protein/Dna interaction. Active orientation of the DnaA protein/dnaA box complex in transcription termination. The Embo Journal 8(5): 1609-1613, 1989

Integrative suppression of dnaA(Ts) mutations mediated by plasmid F in Escherichia coli is a DnaA-dependent process. Molecular and General Genetics 210(2): 262-269, 1987

Specific binding of DnaA protein to a DnaA box in the guaB gene of Escherichia coli K12. European Journal of Biochemistry 241(2): 411-416, 1996

Regulation of expression of the dnaA gene in Escherichia coli: role of the two promoters and the DnaA box. New Biologist 2(6): 574-582, 1990

Transcriptional repression of the dnaA gene of Escherichia coli by dnaA protein. Molecular and General Genetics 209(3): 518-525, 1987

Increased expression of the dnaA gene has no effect on DNA replication in a dnaA+ strain of Escherichia coli. Molecular and General Genetics 192(3): 506-508, 1983

Requirement of the Escherichia coli dnaA gene function for integrative suppression of dnaA mutations by plasmid R 100-1. Molecular and General Genetics 213(1): 163-165, 1988

A temperature sensitive escherichia coli mutant defective in dna replication dnaa a new gene adjacent to the dnaa gene. Molecular & General Genetics 178(3): 541-554, 1980

Genetic organization of the Vibrio harveyi DnaA gene region and analysis of the function of the V. harveyi DnaA protein in Escherichia coli. Journal of Bacteriology 184(9): 2533-2538, 2002