EurekaMag.com logo
+ Site Statistics
References:
53,214,146
Abstracts:
29,074,682
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Transcriptional regulation of muscle-specific genes during myoblast differentiation






Biochemical and Biophysical Research Communications 246(1): 287-292

Transcriptional regulation of muscle-specific genes during myoblast differentiation

A rapid, highly sensitive method to determine the mRNA level of muscle-specific markers using TaqMan PCR analysis was developed and used to study sequential gene regulation of myoblasts during induced differentiation of C2C12 cells. mRNA levels of muscle regulatory factor (MRF) myogenin, alpha-actin, thermoregulatory uncoupling protein UCP2 and glucose transporter isotype glut4 increased rapidly during early stage differentiation. In contrast, myf5, beta-actin, UCP1 and glut1 mRNA levels gradually decreased during 8 days. However, the mRNA level of other genes such as MyoD, glyceraldehyde-3-phosphate dehydrogenase and hexokinase II changed only slightly in comparison. Muscle-specific uncoupling protein UCP3 mRNA was detected during differentiation and increased rapidly within 8 days. These data clearly show the sequential and the differential regulation of muscle-specific genes in C2C12 cells during multinucleate myotube formation.

Accession: 009662101

PMID: 9600108

DOI: 10.1006/bbrc.1998.8600

Download PDF Full Text: Transcriptional regulation of muscle-specific genes during myoblast differentiation



Related references

Transcriptional regulation of ribosomal protein genes during mouse myoblast differentiation. Voellmy, R W , Et Al (Ed ) Icsu (International Council Of Scientific Union) Short Reports, Vol 7 Advances in Gene Technology: The Molecular Biology Of Development; 19th Miami Winter Symposium, Miami, Florida, Usa, February 9-13, Xvii+162p Cambridge University Press: Cambridge, England, Uk; New York, New York, Usa Illus 79, 1987

Myoblast avb3 integrin levels are controlled by transcriptional regulation of expression of the b3 subunit and down-regulation of b3 subunit expression is required for skeletal muscle cell differentiation. Developmental Biology 184: 6-77, 1997

Three linked myosin heavy chain genes clustered within 370 kb of each other show independent transcriptional and post-transcriptional regulation during differentiation of a mouse muscle cell line. Developmental Biology 143(1): 36-43, 1991

Post-transcriptional regulation of ribosome accumulation during myoblast differentiation. Cell 10(4): 587-596, 1977

Transcriptional regulation of ribosomal RNA accumulation during L6E9 myoblast differentiation. Journal of Molecular Biology 134(4): 727-741, 1979

Post transcriptional regulation of 5s ribosomal rna synthesis following myoblast differentiation. European Journal of Biochemistry 184(1): 261-266, 1989

Post-transcriptional regulation of 5S rRNA synthesis following myoblast differentiation. European Journal of Biochemistry 184(1): 261-266, 1989

Myoblast alpha v beta3 integrin levels are controlled by transcriptional regulation of expression of the beta3 subunit and down-regulation of beta3 subunit expression is required for skeletal muscle cell differentiation. Developmental Biology 184(2): 266-277, 1997

Transcriptional regulation of actin and myosin genes during differentiation of a mouse muscle cell line. Differentiation 43(3): 183-191, 1990

Transcriptional and translational regulation of ribosomal protein formation during mouse myoblast differentiation. Journal of Biological Chemistry 262(10): 4868-4875, 1987