Transfer of a benomyl resistance marker by heat-inactivated Trichoderma reesei protoplasts
Meza, V.M.reno, P.T.ngerdy, R.; Gutierrez-Correa, M.
Biotechnology letters 17(8): 827-832
Protoplasts from a benomyl resistant Trichoderma reesei mutant were heat inactivated at 60 degrees C for 8 min and fused with viable protoplasts from an osmosensitive, non-sporulating T. reesei strain. Fusants recovered on 50 micrograms/ml benomyl containing potato dextrose agar plates grew and sporulated well. Cellulolytic enzyme activities produced in liquid culture by selected fusants were higher than those produced by parental strains.