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Transfer of a benomyl resistance marker by heat-inactivated Trichoderma reesei protoplasts

Meza, V.M.reno, P.T.ngerdy, R.; Gutierrez-Correa, M.

Biotechnology letters 17(8): 827-832

1995


ISSN/ISBN: 0141-5492
DOI: 10.1007/bf00129012
Accession: 009663430

Protoplasts from a benomyl resistant Trichoderma reesei mutant were heat inactivated at 60 degrees C for 8 min and fused with viable protoplasts from an osmosensitive, non-sporulating T. reesei strain. Fusants recovered on 50 micrograms/ml benomyl containing potato dextrose agar plates grew and sporulated well. Cellulolytic enzyme activities produced in liquid culture by selected fusants were higher than those produced by parental strains.

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