+ Site Statistics
References:
54,258,434
Abstracts:
29,560,870
PMIDs:
28,072,757
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Transgenic rescue from embryonic lethality and renal carcinogenesis in the Eker rat model by introduction of a wild-type Tsc2 gene



Transgenic rescue from embryonic lethality and renal carcinogenesis in the Eker rat model by introduction of a wild-type Tsc2 gene



Proceedings of the National Academy of Sciences of the United States of America 94(8): 3990-3993



We recently reported that a germ-line insertion in the rat homologue of the human tuberous sclerosis gene (TSC2) gives rise to dominantly inherited cancer in the Eker rat model. In this study, we constructed transgenic Eker rats with introduction of a wild-type Tsc2 gene to ascertain whether suppression of the Eker phenotype is possible. Rescue from embryonic lethality of mutant homozygotes (Eker/Eker) and suppression of N-ethyl-N-nitrosourea-induced renal carcinogenesis in heterozygotes (Eker/+) were both observed, defining the germ-line Tsc2 mutation in the Eker rat as embryonal lethal and tumor predisposing mutation. To the best of our knowledge, this is the first report of rescue from a naturally occurring dominantly inherited cancer. This transgenic rescue system will be useful to analyze Tsc2 gene function, its relation to tumorigenesis in vivo, and genetic-environmental interactions in carcinogenesis.

(PDF emailed within 0-6 h: $19.90)

Accession: 009665773

Download citation: RISBibTeXText

PMID: 9108092

DOI: 10.2307/41943


Related references

Transgenic Rescue From Embryonic Lethality and Renal Carcinogenesis in the Eker Rat Model by Introduction of a Wild-Type Tsc2 Gene. Journal of Urology 159(2): 619-620, 1998

Transgenic rescue from embryonic lethality and renal carcinogenesis in the Nihon rat model by introduction of a wild-type Bhd gene. Oncogene 25(20): 2885-2889, 2005

Presence of a modifier gene(s) affecting early renal carcinogenesis in the Tsc2 mutant (Eker) rat model. International Journal of Oncology 24(1): 75-80, 2003

Overexpression of members of the AP-1 transcriptional factor family from an early stage of renal carcinogenesis and inhibition of cell growth by AP-1 gene antisense oligonucleotides in the Tsc2 gene mutant (Eker) rat model. Biochemical and Biophysical Research Communications 241(1): 24-30, 1998

Molecular genetic basis of renal carcinogenesis in the Eker rat model of tuberous sclerosis (Tsc2). Molecular Carcinogenesis 14(1): 23-27, 1995

Renal carcinogenesis, hepatic hemangiomatosis, and embryonic lethality caused by a germ-line Tsc2 mutation in mice. Cancer Research 59(6): 1206-1211, 1999

Identification of cDNAs induced by the tumor suppressor Tsc2 gene using a conditional expression system in Tsc2 mutant (Eker) rat renal carcinoma cells. Biochemical and Biophysical Research Communications 247(3): 728-733, 1998

Mapping and determination of the cDNA sequence of the Erc gene preferentially expressed in renal cell carcinoma in the Tsc2 gene mutant (Eker) rat model. Biochemical and Biophysical Research Communications 275(1): 134-140, 2000

Multistep Renal Carcinogenesis in the Eker (Tsc 2 Gene Mutant) Rat Model. Current Molecular Medicine 4(8): 807-811, 2004

The presence of modifier gene in renal carcinogenesis in the Eker rat model. Proceedings of the American Association for Cancer Research Annual Meeting 38(0): 468, 1997

Multistep renal carcinogenesis in the Eker (Tsc 2 gene mutant) rat model. Current Molecular Medicine 4(8): 807-811, 2004

Use of the spontaneous Tsc2 knockout (Eker) rat model of hereditary renal cell carcinoma for the study of renal carcinogens. Toxicologic Pathology 30(6): 675-680, 2002

Distribution of Tsc2 protein in various normal rat tissues and renal tumours of Tsc2 mutant (Eker) rat detected by immunohistochemistry. Virchows Archiv 434(4): 341-350, 1999

Suppression of cytochrome P450 1A1 and 4A1 gene expression in renal carcinomas of TSC2 gene mutant (Eker) rats. International Journal of Oncology 18(1): 147-149, 2000

The transcriptional profile of the kidney in Tsc2 heterozygous mutant Long Evans (Eker) rats compared to wild-type. Mutation Research 549(1-2): 213-224, 2004