+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Translation but not the encoded sequence is essential for the efficient propagation of the defective interfering RNAs of the coronavirus mouse hepatitis virus

Translation but not the encoded sequence is essential for the efficient propagation of the defective interfering RNAs of the coronavirus mouse hepatitis virus

Journal of Virology 69(6): 3744-3751

The defective interfering (DI) RNA MIDI of mouse hepatitis virus strain A59 (MHV-A59) contains a large open reading frame (ORF) spanning almost its entire genome. This ORF consists of sequences derived from ORF1a, ORF1b, and the nucleocapsid gene. We have previously demonstrated that mutations that disrupt the ORF decrease the fitness of MIDI and its derivatives (R. J. de Groot, R. G. van der Most, and W. J. M. Spaan, J. Virol. 66:5898-5905, 1992). To determine whether translation of the ORF per se is required or whether the encoded polypeptide or a specific sequence is involved, we analyzed sets of related DI RNAs containing different ORFs. After partial deletion of ORF1b and nucleocapsid gene sequences, disruption of the remaining ORF is still lethal; translation of the entire ORF is not essential, however. When a large fragment of the MHV-A59 spike gene, which is not present in any of the MHV-A59 DI RNAs identified so far, was inserted in-frame into a MIDI derivative, translation across this sequence was vital to DI RNA survival. Thus, the translated sequence is irrelevant, indicating that translation per se plays a crucial role in DI virus propagation. Next, it was examined during which step of the viral life cycle translation plays its role. Since the requirement for translation also exists in DI RNA-transfected and MHV-infected cells, it follows that either the synthesis or degradation of DI RNAs is affected by translation.

Please choose payment method:

(PDF emailed within 1 workday: $29.90)

Accession: 009667375

Download citation: RISBibTeXText

PMID: 7745722

Related references

Replication of synthetic defective interfering RNAs derived from coronavirus mouse hepatitis virus-A59. Virology 216(1): 174-183, 1996

Structure of the intracellular defective viral RNAs of defective interfering particles of mouse hepatitis virus. Journal of Virology 54(2): 329-336, 1985

A domain at the 3' end of the polymerase gene is essential for encapsidation of coronavirus defective interfering RNAs. Journal of Virology 65(6): 3219-3226, 1991

Expression of hemagglutinin/esterase by a mouse hepatitis virus coronavirus defective-interfering RNA alters viral pathogenesis. Virology 242(1): 170-183, 1998

A subgenomic mRNA transcript of the coronavirus mouse hepatitis virus strain A59 defective interfering (DI) RNA is packaged when it contains the DI packaging signal. Journal of Virology 71(7): 5684-5687, 1997

Subgenomic RNA synthesis directed by a synthetic defective interfering RNA of mouse hepatitis virus: a study of coronavirus transcription initiation. Journal of Virology 68(6): 3656-3666, 1994

Coronavirus defective-interfering RNA as an expression vector: the generation of a pseudorecombinant mouse hepatitis virus expressing hemagglutinin-esterase. Virology 208(1): 319-327, 1995

Defective-interfering particles of murine coronavirus: mechanism of synthesis of defective viral RNAs. Virology 163(1): 104-111, 1988

Deletion mapping of a mouse hepatitis virus defective interfering RNA reveals the requirement of an internal and discontiguous sequence for replication. Journal of Virology 67(10): 6110-6118, 1993

Sequence analysis of cymbidium ringspot virus satellite and defective interfering RNAs. Journal of General Virology 71: 1655-1660, 1990

Sequence and structure of defective interfering RNAs associated with cucumber necrosis virus infections. Journal of General Virology 74: 1715-1720, 1993

Mouse hepatitis virus S RNA sequence reveals that nonstructural proteins ns4 and ns5a are not essential for murine coronavirus replication. Journal of Virology 65(10): 5605-5608, 1991

Structural requirements in an essential segment of tomato bushy stunt virus defective interfering RNAS. 1995

Assembled coronavirus from complementation of two defective interfering RNAs. Journal of Virology 71(5): 3922-3931, 1997

Coat protein of cucumber necrosis virus is not required for efficient generation or accumulation of defective interfering RNAs. Journal of General Virology 75: 2505-2508, 1994