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Transpositional activity of IS986 in Mycobacterium smegmatis

Transpositional activity of IS986 in Mycobacterium smegmatis

Gene (Amsterdam) 130(1): 99-105

The insertion sequence IS986 (also known as IS6110) has been widely used for typing Mycobacterium tuberculosis isolates, due to the extensive multiple polymorphism shown using this probe. Although this polymorphism is presumed to be due to the mobility of IS986, transposition of this element has not previously been demonstrated in the laboratory. Using artificial composite transposons constructed in a vector unable to replicate in mycobacteria, we have succeeded in demonstrating the mobility of IS986 in M. smegmatis, apparently through cointegrate formation and transposition. The apparently random nature of IS986 insertions in M. tuberculosis is advantageous for transposon mutagenesis, although its efficient use will require more effective delivery systems.

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Accession: 009669733

Download citation: RISBibTeXText

PMID: 8393827

DOI: 10.1016/0378-1119(93)90351-3

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