+ Site Statistics
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on LinkedInFollow on LinkedIn

+ Translate

Two-stage methanotrophic bioreactor for the treatment of chlorinated organic wastewater

Two-stage methanotrophic bioreactor for the treatment of chlorinated organic wastewater

Water Research 31(8): 2026-2036

A two-stage continuous methanotrophic bioreactor was developed for the treatment of wastewater contaminated by chlorinated organic solvents. The chosen design eliminated the problem of competitive inhibition during cometabolic biodegradations by separating the consumption of growth substrate (methane) and the degradation of chlorinated organics into two stages. In the first stage, a mixed methanotrophic culture was grown in a dispersed-growth continuous flow stirred tank reactor (CFSTR). In the second stage, trichloroethylene (TCE) and/or cis-1,2-dichloroethylene (cDCE) contaminated wastewater was mixed with the suspended cells from the growth reactor and fed into a plug-flow reactor (PFR) where the cometabolic degradation occurred. The mixed methanotrophic culture in the CFSTR was grown in a copper-free, iron-enriched nitrate mineral salts medium to induce methanotrophic cells to produce soluble methane monooxygenase enzymes which are highly active in cometabolic degradations. Formate and oxygen were added prior to the PFR to enhance the chlorinated organic degradation rates and capacities. A kinetic model that incorporates chlorinated organic transformation capacity and competitive inhibition was used to develop the reactor design and to predict treatment performance for TCE and/or cDCE. Model predictions were verified by comparisons with experimental data. A bench-scale two-stage reactor (with a 4-h wastewater retention time) was demonstrated to be capable of treating wastewater mixtures containing TCE (4.7 mg/1) and cDCE (4.8 mg/1) to below the maximum contaminant levels (MCLs, 5 mu-g/l each) continuously for at least 31 d. The optimal overall material cost of methanotrophic cell growth, formate amendment, and oxygen amendment for the treatment of TCE and cDCE wastewater (5 mu-g/l each) to the MCLs was estimated to be 0.17 per 1000 liters of wastewater.

(PDF emailed within 0-6 h: $19.90)

Accession: 009687157

Download citation: RISBibTeXText

DOI: 10.1016/s0043-1354(97)00020-1

Related references

Enrichment of denitrifying methanotrophic bacteria from municipal wastewater sludge in a membrane bioreactor at 20°C. Journal of Hazardous Materials 274: 428-435, 2015

Removal of pharmaceuticals and organic matter from municipal wastewater using two-stage anaerobic fluidized membrane bioreactor. Bioresource Technology 165: 42-49, 2015

Methanotrophic TCE Biodegradation in a Multi-Stage Bioreactor. Environmental Science & Technology 29(8): 2073-2082, 1995

Methanotrophic cometabolism of trichloroethylene tce in a two stage bioreactor system. Water Research 26(2): 259-265, 1992

Post-treatment of secondary wastewater treatment plant effluent using a two-stage fluidized bed bioreactor system. Journal of Environmental Health Science & Engineering 11(1): 10-10, 2014

Development of multi stage reversing flow bioreactor mrb for wastewater treatment. Water Science & Technology 20(11-12): 361-368, 1988

Developing of multi-stage reversing-flow bioreactor (MRB) for wastewater treatment. Water Science and Technology 20(11-12): 361-367, 1988

Pilot-scale demonstration of a two-stage methanotrophic bioreactor for biodegradation of trichloroethylene in groundwater. Journal of the Air and Waste Management Association 45(1): 12-19, 1995

Two-stage anaerobic fluidized-bed membrane bioreactor treatment of settled domestic wastewater. Water Science and Technology 68(2): 394-399, 2013

Treatment of chlorinated organic materials containing wastewater by oxidation processes. Fresenius Environmental Bulletin 9(9/10): 590-596, 2000

Characterization of dissolved organic matter in a dynamic membrane bioreactor for wastewater treatment. Chinese Science Bulletin 58(15): 1717-1724, 2013

Treatment of urban wastewater in a membrane bioreactor at high organic loading rates. Journal of Biotechnology 92(2): 95-101, 28 December, 2001

High rate treatment of terephthalic acid production wastewater in a two-stage anaerobic bioreactor. Biotechnology and Bioengineering 91(2): 169-179, 2005

Removal of chlorinated organic compounds during wastewater treatment: achievements and limits. Applied Microbiology and Biotechnology 98(14): 6233-6242, 2015

Organic stabilization and nitrogen removal in membrane separation bioreactor for domestic wastewater treatment. Water Science & Technology 25(10): 231-240, 1992