+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Tyrosine hydroxylase phosphorylation in bovine adrenal chromaffin cells: The role of intracellular Ca-2+ in the histamine H-1 receptor-stimulated phosphorylation of Ser-8, Ser-19, Ser-31, and Ser-40



Tyrosine hydroxylase phosphorylation in bovine adrenal chromaffin cells: The role of intracellular Ca-2+ in the histamine H-1 receptor-stimulated phosphorylation of Ser-8, Ser-19, Ser-31, and Ser-40



Journal of Neurochemistry 64(3): 1370-1378



Tyrosine hydroxylase (TOH), the rate-limiting enzyme in catecholamine biosynthesis, is regulated by phosphorylation. Activation of histaminergic H-1 receptors on cultured bovine adrenal chromaffin cells stimulated a rapid increase in TOH phosphorylation (within 5 s) that was sustained for at least 5 min. The initial increase in TOH phosphorylation (up to 1 min) was essentially unchanged by the removal of extracellular Ca-2+. In contrast, the H-1-mediated response was abolished by preloading the cells with BAPTA acetoxymethyl ester (50 mu-M) and significantly reduced by prior exposure to caffeine (10 mM for 10 min) to deplete intracellular Ca-2+. Tryptic-phosphopeptide analysis by HPLC revealed that the H-1 response in the presence or absence of extracellular Ca-2+ resulted in a major increase in the phosphorylation of Ser-19 with smaller increases in that of Ser-40 and Ser-31 In contrast, although a brief stimulation with nicotine (30 mu-M for 60 s) also resulted in a major increase in Ser-19 phosphorylation, this response was abolished in the absence of extracellular Ca-2+. These data indicate that the mobilization of intracellular Ca-2+ plays a crucial role in supporting H-1-mediated TOH phosphorylation and may thus have a potentially important role in regulating catecholamine synthesis.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 009688539

Download citation: RISBibTeXText

PMID: 7861170

DOI: 10.1046/j.1471-4159.1995.64031370.x


Related references

Protein phosphorylation in bovine adrenal medullary chromaffin cells: histamine-stimulated phosphorylation of tyrosine hydroxylase. Journal of Neurochemistry 59(1): 164-174, 1992

Potentiation by apamin of histamine-stimulated catecholamine biosynthesis and tyrosine hydroxylase phosphorylation in cultured bovine adrenal chromaffin cells. Tokushima Journal of Experimental Medicine 43(1-2): 17-23, 1996

Tyrosine hydroxylase phosphorylation in bovine adrenal chromaffin cells: Clonidine stimulates basal but inhibits nicotinic receptor evoked phosphorylation. Biochemical Pharmacology 51(3): 239-245, 1996

Angiotensin II-stimulated tyrosine hydroxylase activity and phosphorylation in bovine adrenal chromaffin cells. Journal of Neurochemistry 74(Suppl ): S38, 2000

Intracellular calcium-dependent phosphorylation of tyrosine hydroxylase in bovine adrenal chromaffin cells. Journal of Neurochemistry 65(Suppl. ): S5, 1995

Tyrosine hydroxylase in bovine adrenal chromaffin cells: Angiotensin II-stimulated activity and phosphorylation of Ser19, Ser31, and Ser40. Journal of Neurochemistry 70(6): 2565-2573, 1998

Tyrosine hydroxylase phosphorylation in bovine adrenal chromaffin cells: the role of MAPKs after angiotensin II stimulation. Journal of Neurochemistry 78(3): 490-498, 2001

Cholinergic receptor-mediated phosphorylation and activation of tyrosine hydroxylase in cultured bovine adrenal chromaffin cells. Journal of Neurochemistry 46(2): 610-622, 1986

Tyrosine hydroxylase phosphorylation in digitonin-permeabilized bovine adrenal chromaffin cells: The effects of protein kinase and phosphatase inhibitors on Ser19 and Ser40 phosphorylation. Journal of Neurochemistry 69(6): 2387-2396, 1997

Histamine-stimulated phospholipase C signalling in the adrenal chromaffin cell: effects on inositol phospholipid metabolism and tyrosine hydroxylase phosphorylation. Clinical and Experimental Pharmacology & Physiology 24(8): 624-631, 1997

Histaminergic phosphorylation of tyrosine hydroxylase in bovine adrenal chromaffin cells. Journal of Neurochemistry 61(Suppl. ): S153, 1993

Hierarchical phosphorylation of tyrosine hydroxylase in bovine adrenal chromaffin cells. Journal of Neurochemistry 88(Suppl. 1): 82, 2004

Tyrosine hydroxylase phosphorylation in digitonin-permeabilised bovine adrenal chromaffin cells. Journal of Neurochemistry 69(Suppl. ): S207, 1997

Phosphorylation profile of soluble and particulate tyrosine hydroxylase in bovine adrenal chromaffin cells. Society for Neuroscience Abstract Viewer & Itinerary Planner : Abstract No 742 15, 2002

Phorbol ester-mediated phosphorylation of tyrosine hydroxylase in bovine adrenal chromaffin cells. Journal of Neurochemistry 72(Suppl. ): S25, 1999