+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Unpredictability of triphenyltetrazolium chloride in staining irreversible ischaemia-reperfusion injury in the skeletal muscle of rats

Unpredictability of triphenyltetrazolium chloride in staining irreversible ischaemia-reperfusion injury in the skeletal muscle of rats

European Journal of Surgery 162(5): 407-412

Objective: To find out whether pale staining with triphenyltetrazolium chloride (TTC) in skeletal muscle of rat hindlimbs which had been subjected to ischaemia-reperfusion definitely indicated irreversible tissue damage. Design: Laboratory study. Setting: University hospital, Taiwan. Material: 77 Female Wistar rats. Interventions: Ischaemia of one hindlimb was caused by wrapping of a tourniquet above knee joint for 1.5 (n = 14), 2 (n 15), 2.5 (n = 17), 3 (n = 17) or 4 (n = 14) hours. Each ischemic group was divided into three subgroups to receive nil, I or 1.5 hours reperfusion, respectively. Gastrocnemius and solcus muscles were excised bilaterally. Main outcome measures: TTC reduction in the ischemic limbs presented as a percentage of the opposite control limb measured by a spectrophotometric assay. Density of red formazan deposition in the ischemic limbs assessed by microscopic examination of the TTC histochemical stain in the 3 hour ischemic group. Results: In the 2, 2.5, and 3 hour ischemic groups the TTC reduction in the ischemic limbs after 1.5 hours reperfusion increased significantly as compared with that with one hour reperfusion (p lt 0.01 in each case). The density of red formazan deposition in the muscle after 3 hours ischaemia and 1.5 hours reperfusion was significantly higher than that of only 1 hour of reperfusion. Conclusion: Lack of TTC staining does not necessarily represent irreversible ischaemia-reperfusion injury of the skeletal muscle in rats.

Please choose payment method:

(PDF emailed within 1 workday: $29.90)

Accession: 009696730

Download citation: RISBibTeXText

PMID: 8781924

Related references

Triphenyltetrazolium staining of irreversible ischemic injury following coronary artery occlusion in rats. American Journal of Pathology 121(3): 522-530, 1985

Reperfusion injury in skeletal muscle: controlled limb reperfusion reduces local and systemic complications after prolonged ischaemia. Cardiovascular Surgery 2(6): 737-748, 1994

Preconditioning of skeletal muscle against ischaemia/reperfusion injury. Clinical Science 92(2): 8P, 1997

The importance of reperfusion on determining infarct size with triphenyltetrazolium chloride staining in rat hearts. Journal of Molecular & Cellular Cardiology 24(Suppl. 1): S47, 1992

Do prostaglandins have a salutary role in skeletal muscle ischaemia-reperfusion injury?. European Journal of Vascular and Endovascular Surgery 18(5): 439-444, 1999

Nrf2 augments skeletal muscle regeneration after ischaemia-reperfusion injury. Journal of Pathology 234(4): 538-547, 2014

Skeletal muscle ischaemia-reperfusion injury: further characterisation of a rodent model. European Journal of Vascular and Endovascular Surgery 22(6): 523-527, 2001

What is the required reperfusion period for assessment of myocardial infarct size using triphenyltetrazolium chloride staining in the rat?. Journal of Thrombosis and Thrombolysis 10(2): 181-187, 2000

The role of mast cells in ischaemia-reperfusion injury in murine skeletal muscle. Journal of Pathology 191(4): 443-448, 2000

Mild hypothermia protects against ischaemia-reperfusion injury in rabbit skeletal muscle. British Journal of Plastic Surgery 50(5): 343-348, 1997

Oral vitamin C attenuates acute ischaemia-reperfusion injury in skeletal muscle. Journal of Bone and Joint Surgery. British Volume 83(8): 1202-1206, 2001

Early assessment of skeletal muscle damage after ischaemia-reperfusion injury using Tc-99m-glucarate. Cardiovascular Surgery 8(3): 186-191, 2000

Ischaemia-reperfusion modulates inflammation and fibrosis of skeletal muscle after contusion injury. International Journal of Experimental Pathology 91(3): 244-255, 2010

The effect of surgical and chemical denervation on ischaemia/reperfusion injury of skeletal muscle. Journal of Plastic Reconstructive and Aesthetic Surgery 65(2): 240-248, 2012