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Unpredictability of triphenyltetrazolium chloride in staining irreversible ischaemia-reperfusion injury in the skeletal muscle of rats



Unpredictability of triphenyltetrazolium chloride in staining irreversible ischaemia-reperfusion injury in the skeletal muscle of rats



European Journal of Surgery 162(5): 407-412



Objective: To find out whether pale staining with triphenyltetrazolium chloride (TTC) in skeletal muscle of rat hindlimbs which had been subjected to ischaemia-reperfusion definitely indicated irreversible tissue damage. Design: Laboratory study. Setting: University hospital, Taiwan. Material: 77 Female Wistar rats. Interventions: Ischaemia of one hindlimb was caused by wrapping of a tourniquet above knee joint for 1.5 (n = 14), 2 (n 15), 2.5 (n = 17), 3 (n = 17) or 4 (n = 14) hours. Each ischemic group was divided into three subgroups to receive nil, I or 1.5 hours reperfusion, respectively. Gastrocnemius and solcus muscles were excised bilaterally. Main outcome measures: TTC reduction in the ischemic limbs presented as a percentage of the opposite control limb measured by a spectrophotometric assay. Density of red formazan deposition in the ischemic limbs assessed by microscopic examination of the TTC histochemical stain in the 3 hour ischemic group. Results: In the 2, 2.5, and 3 hour ischemic groups the TTC reduction in the ischemic limbs after 1.5 hours reperfusion increased significantly as compared with that with one hour reperfusion (p lt 0.01 in each case). The density of red formazan deposition in the muscle after 3 hours ischaemia and 1.5 hours reperfusion was significantly higher than that of only 1 hour of reperfusion. Conclusion: Lack of TTC staining does not necessarily represent irreversible ischaemia-reperfusion injury of the skeletal muscle in rats.

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Accession: 009696730

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PMID: 8781924


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