+ Site Statistics
References:
52,654,530
Abstracts:
29,560,856
PMIDs:
28,072,755
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Usage of T cell receptor beta-chain variable gene is highly restricted at the site of inflammation in murine autoimmune uveitis



Usage of T cell receptor beta-chain variable gene is highly restricted at the site of inflammation in murine autoimmune uveitis



Journal of Immunology 150(12): 5716-5721



Improved molecular methods allow identification of the specific autoaggressive T cells involved in auto immune inflammations. In this study of interphotoreceptor retinoid-binding protein-induced uveitis, TCR V-beta usage was studied using RNA-polymerase chain reaction amplification of transcripts derived directly from ocular tissues and from T cell lines obtained from the spleen. Specific V-beta-5' primers from the major murine TCR V-beta families were coupled with a common 3' primer from the V-beta C region. Amplification of rearranged TCR V-beta-D-beta-J-beta-C-beta sequences was confirmed by Southern blot analysis. In ocular tissue from sensitized mice, TCR V-beta expression was limited mainly to one to three V-beta families, with predominant expression of V-beta-2, V-beta-12, and V-beta-15. In most animals there was similar, albeit limited, TCR gene usage in both the recognition of autoantigen in uveitogenic T cell lines and at the site of inflammation in the eye. Identification of a limited TCR V-beta repertoire in Ag-reactive T cell lines correlated with TCR usage at the target site of autoimmune expression. The gene products of the restricted TCR V-beta rearrangements found in lesions and in the cell lines may serve as the target for selective immunotherapy.

(PDF emailed within 1 workday: $29.90)

Accession: 009702146

Download citation: RISBibTeXText

PMID: 8390540


Related references

Usage of T cell receptor b-chain variable gene is highly restricted at the site of inflammation in murine autoimmune uveitis. Journal of Immunology 150: 16-21, 1993

T cell receptor beta chain gene rearrangement shared by murine T cell lines derived from a site of autoimmune inflammation. Journal of Clinical Investigation 81(6): 1810-1818, 1988

Restricted usage of T-cell receptor alpha-chain variable region (TCRAV) and T-cell receptor beta-chain variable region (TCRBV) repertoires after human allogeneic haematopoietic transplantation. British Journal of Haematology 109(4): 759-769, 2000

Restricted usage of T-cell receptor alpha-chain variable region and T-cell receptor beta-chain variable region repertoires after human allogeneic haematopoietic transplantation. British Journal of Haematology 109(4): 759-769, June, 2000

T cell receptor beta-chain third complementarity-determining region gene usage is highly restricted among Sm-B autoantigen-specific human T cell clones derived from patients with connective tissue disease. Arthritis and Rheumatism 42(4): 703-709, 1999

Restricted V-beta T-cell receptor usage in murine collagen induced autoimmune ear disease. Journal of Allergy & Clinical Immunology 99(1 PART 2): S174, 1997

Lack of restricted T-cell receptor beta-chain variable region (V beta) usage of reactive T-lymphocytes in Hodgkin's disease. British Journal of Haematology 86(3): 524-532, 1994

T-cell receptor (TCR) usage in Lewis rat experimental autoimmune encephalomyelitis: TCR beta-chain-variable-region V beta 8.2-positive T cells are not essential for induction and course of disease. Proceedings of the National Academy of Sciences of the United States of America 92(13): 5850-5854, 1995

Increased usage of T-cell receptor beta-chain variable region gene families V-beta-2, V-beta-4, and V-beta-5. Pediatric Research 35(4 PART 2): 17A, 1994

Restricted usage of T-cell receptor α-chain variable region (TCRAV) and T-cell receptor β-chain variable region (TCRBV) repertoires after human allogeneic haematopoietic transplantation. British Journal of Haematology 109(4): 759-769, 2000

Evidence for highly restricted T cell receptor V-beta gene usage by CSF lymphocytes in multiple sclerosis. Australian & New Zealand Journal of Medicine 22(4): 420, 1992

Highly biased CDR3 usage in restricted sets of beta chain variable regions during viral superantigen 9 response. Journal of Experimental Medicine 187(2): 253-258, 1998

Preferential t cell receptor beta chain variable gene usage in pulmonary sarcoidosis. American Review of Respiratory Disease 145(4 PART 2): A414, 1992

T-cell receptor beta-chain variable region gene usage in umbilical cord lymphocytes. Pediatric Research 33(4 PART 2): 287A, 1993

Skewed T-cell receptor beta chain variable gene (TCRBV) usage among different clinical types of patients with chronic HBV infection. Fems Immunology and Medical Microbiology 65(3): 448-455, 2013