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Use of Hoechst fluorescence dye 33258 for quantitative estimation of intracellular anthracycline binding to DNA



Use of Hoechst fluorescence dye 33258 for quantitative estimation of intracellular anthracycline binding to DNA



Antibiotiki i Khimioterapiya 37(5): 21-24



The paper deals with development of a procedure for quantitative determination of the share of anthracycline antibiotics bound in cells directly to DNA. A DNA-specific Hoechst fluorescence dye 33258 was used for the purpose. The level of its quenching on DNA correlated with the quantity of the antibiotic bound to it. It was shown that the quenching of the Hoechst fluorescence dye bound to DNA was not due to the dye competition with the antibiotic for the site of bounding on DNA, as was suggested earlier. It was likely to be defined by reabsorption of the radiation by antibiotic molecules.

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Accession: 009702544

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