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Use of micellar mobile phases and an HPLC column switching system for direct injection determination of urinary free cortisol



Use of micellar mobile phases and an HPLC column switching system for direct injection determination of urinary free cortisol



Journal of Liquid Chromatography 16(12): 2583-2594



A direct injection method for the determination of urinary free cortisol by an HPLC column switching system is presented. The first chromatographic column, which provides for sample preconcentration and clean-up, employs a micellar mobile phase. The second column, coupled on -line to the first, utilizes reversed-phase condition for analysis. UV detection is employed at 254 nm. Detection limit for urinary free cortisol is 2.4 ng when 2 ml urine sample is injected. Nine samples were analyzed. The results were in a reasonable range. This method can be used successfully in the diagnosis of patients with hypercortisolism and Cushing's syndrome.

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Accession: 009705930

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DOI: 10.1080/10826079308019594


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