EurekaMag.com logo
+ Site Statistics
References:
48,253,228
Abstracts:
22,798,842
+ Resolve Accession
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
Submit PDF Full TextSubmit PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Utilization of side-chain precursor for penicillin biosynthesis in a high-producing strain of Penicillium chrysogenum


, : Utilization of side-chain precursor for penicillin biosynthesis in a high-producing strain of Penicillium chrysogenum. Applied Microbiology & Biotechnology 40(6): 883-887

Utilization of the side-chain precursors phenoxyacetic acid (POA) and phenylacetic acid (PA) for penicillin biosynthesis by Penicillium chrysogenum was studied in shake flasks. Precursor uptake and penicillin production were followed by HPLC analysis of precursors and products in the medium and in the cells. P. chrysogenum used both POA and PA as precursors, producing phenoxymethylpenicillin (penicillin V) and benzylpenicillin (penicillin G), respectively. If both precursors were present simultaneously, the formation of penicillin V was blocked and only penicillin G was produced. When PA was added at different times to cells that were induced initially for POA utilization and were producing penicillin V, the POA utilization and penicillin V formation were blocked, whereas the cells started utilizing PA and produced penicillin G. The blocking of the POA turnover lasted for as long as PA was present in the medium. If POA was added to cultures induced initially for PA utilization and producing penicillin G, this continued irrespective of the presence of POA. Utilization of POA increased concomitant with depletion of PA from the medium. Analysis of cellular pools from a growing cell system with POA as precursor to which PA was added after 48 h showed that the cellular concentration of POA was kept high without production of penicillin V and at a concentration comparable to the concentration in the medium. The cellular concentration of POA was higher than the concentration of PA that was utilized for penicillin G production.

(PDF 0-2 workdays service)

Order PDF Full Text

Close
Close

Click here to order any other PDF Full Text


Accession: 009711088

PDF Full Text: Submit


Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:
Close
Close

Other references

Barchas, J.; Erdelyi, E.; Angwin, P., 1972: Simultaneous determination of indole- and catecholamines in tissues using a weak cation-exchange resin. The method was used to estimate noradrenaline, adrenaline, dopamine, serotonin and 5-methoxytryptamine.

Blakely, RL., 1981: Formulating an exhibit philosophy. International Zoo Yearbook, 21: 1-5

Elias, P. M.; Goerke, J.; Friend, D. S., 1977: Mammalian epidermal barrier layer lipids composition and influence on structure. The outer layers of the mammalian epidermis protect the organism from H2O loss and external injury. The barrier as visualized with tracers was shown to develop initially in the stratum granulosum concurrent with the intercellular deposition of ext...

Katerndahl, D.A., 1992: Natural history of phobic anxiety. The characteristics of those with phobic anxiety--anxiety with or without avoidance of specific fear-provoking situations--seen in family practice have not been previously described. However, patients with agoraphobia--extreme avoidance of fear-pr...

French, Sherrie, 1976: A touch of broadway comes to Bryan, Ohio. A script for a nutrition skit and a short story are prefaced by a short article on the author. The playwright is an eighth grade student from Bryan, Ohio. She is a member of a group called Youthpower, which provides direct communication between st...

Lundin, Richard, 2001: Erosion by the solar wind. In this issue, Seki et al. describe erosion of Earth's atmosphere by the solar wind. A substantial difference in the rate of atmospheric and hydrospheric erosion between the 4 Earth-like planets of the solar system provides an important part...

Winnitzky, I., 1932: Untersuchungen uber den Einfluss gesteigerter Kalziumzufuhr auf den Befall mit Askariden bei Tieren im besonderen Hinblick auf die Pelztierzucht, sowie uber die Moglichkeit der ausschliesslichem Fleischfutterung der Karnivoren unter gleichzeitiger Zugabe eines Kalkvitaminpraparates. Z Pelztier Rauchwarenkunde Leipzig, 4: 79-143

Franklin R.B.; Qian K.; Costello L.C., 1990: Regulation of aspartate aminotransferase messenger rna level by testosterone. The effect of testosterone on precursor mitochondrial aspartate aminotransferase (pmAAT) mRNA was studied in rat ventral prostate and primary cell cultures of mini-pig prostate. Testosterone induced a 2-3 fold increase in pmAAT mRNA level in both...

Jewell, F. F., 1960: Inoculation of slash pine seedlings with Cronartium fusiforme. Techniques have been developed for inoculating large numbers of pine seedlings with fusiform rust. Cotyledonary slash pine seedlings in nursery beds were inoculated by placing telia-bearing oak leaves over them and maintaining high humidity for 72...

Bonaminio, V., 2013: Response by Vincenzo Bonaminio. International Journal of Psycho-Analysis 94(1): 124-127