A Gb-like protein from soybean
Nielsen, N.C.; Beilinson, V.; Bassuner, R.
Physiologia Plantarum 111(1): 82
2001
ISSN/ISBN: 0031-9317 Accession: 009746114
A cDNA clone that encodes a Gb-like protein was isolated from germinating soybeans. The deduced amino acid sequence for this protein is similar to other Gb-like proteins isolated from plants and animals and contains 7 copies of the WD-40 repeat. RNA blots and reverse transcriptase polymerase chain reaction (PCR) revealed that mRNA for the soybean Gb-like protein is present during seed development and germination, periods during seed ontogeny that are associated with high levels of biosynthetic activity. DNA blot analysis suggested there are only one or two copies of this gene in the soybean genome. Antibodies were raised against recombinant soybean Gb-like protein expressed in bacteria. The antibodies reacted with a 35-kDa soybean protein that was present in minor amounts in developing seeds. The precise functions of Gb-like proteins are unknown, but there are indications that they are involved in signal transduction pathways and in this way participate in control of seed growth and development. Reprinted by permission of the publisher.