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A new intragenic polymorphism detected by the single-strand conformation polymorphism (SSCP) assay in the dystrophin gene



A new intragenic polymorphism detected by the single-strand conformation polymorphism (SSCP) assay in the dystrophin gene



Human Mutation 1(3): 221-223



We have employed the single strand conformation polymorphism (SSCP) technique to examine a group of patients with Duchenne or Becker muscular dystrophy who do not contain deletions detectable by multiplex PCR or Southern/cDNA, in an attempt to identify uncommon mutations within the dystrophin gene. In SSCP analysis, a mutated sequence can be detected as a change of mobility in a nondenaturing polyacrylamide gel. During the course of this investigation, we detected and characterized a new polymorphism at the 3' end of intron 16. The G-to-T base change creates a TaqI restriction site which allows for rapid typing of the polymorphism by restriction digestion and electrophoresis of PCR amplified products. Its localization inside the 5' region of the dystrophin gene and its high heterozygosity makes it a useful and easy tool for rapid carrier and prenatal diagnosis.

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Accession: 009752348

Download citation: RISBibTeXText

PMID: 1301928

DOI: 10.1002/humu.1380010308


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