EurekaMag.com logo
+ Site Statistics
References:
47,893,527
Abstracts:
28,296,643
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Anti-tolA antibody in non-A, non-B chronic liver disease






Fukuoka Igaku Zasshi 83(10): 367-376

Anti-tolA antibody in non-A, non-B chronic liver disease

We constructed a cDNA library against the plasma obtained from the patient with acute exacerbation of non-A, non-B liver cirrhosis, and immunoscreened this library with the sera obtained from the patients with non-A, non-B chronic liver disease. One positive clones lambda-22C containing about 1.2 kb cDnA insert was isolated from 10-6 clones. Nucleotide sequence determination and subsequent homology search revealed its identity to the tolA gene of Escherichia coli. Anti-tolA antibody was detected in 54.5% of the patients with NANB chronic liver disease whose sera was negative for antibody to hepatitis C virus (anti-C100). In contrast, anti-tolA was detected only of 14.6% patients with anti-C100 positive NANB chronic liver disease, 10.5% with hepatitis B surface antigen-positive chronic liver disease, 7.7% with alcoholic liver disease and 4.2% in normal control, and non positive case in acute hepatitis of etiology and in primary biliary cirrhosis. However, antibody to the core protein of hepatitis C virus (anti-JCC) was detected 50% of the patients whose sera were negative for anti-C100 but positive for anti-tolA. Recently, it has been reported that hepatitis C virus is rich in mutations and has some variants. These results indicated the presence of a common epitope between the tolA protein and some agent related to non-A, non-B hepatitis, especially to anti-C100 negative non-A, non-B hepatitis such as variants of hepatitis C virus which have mutations in C100 coded region.


Accession: 009770189

PMID: 1281459



Related references

Pramoolsinsap, C.; Kurathong, S.; Lerdverasirikul, P., 1992: Prevalence of anti-HCV antibody in family members of anti-HCV-positive patients with acute and chronic liver disease. Presence of circulating anti-hepatitis C antibody (anti-HCV) was screened in 201 Thai patients with acute and chronic liver disease who presented to Ramathibodi and Phya Thai Hospitals during 1984-1990. Of these, 29 patients (14.4%) were positive...

Takaki, A.; Sakaguchi, K.; Ogawa, S.; Kawamoto, H.; Tsuji, T., 1994: Specificities and clinical significance of anti-cytoskeleton antibodies in anti-smooth muscle antibody-positive patients with chronic liver disease C. We investigated the specificities and characteristics of anti-cytoskeleton antibodies in 13 anti-smooth muscle antibody (ASMA)-positive patients with chronic liver disease C (CLD-C), and compared them with those in 7 ASMA-positive patients with au...

Macedo, G.; Carvalho, L.; Bodas, A.; Vale, A.M.H.; Sarmento, J.A.; Ribeiro, T., 1995: Anti-neutrophil cytoplasmic antibody in chronic liver disease. Gastroenterology 108(4 SUPPL ): A1114

Stransky J.; Nemecek V.; Konig J., 1990: Prevalence of anti hcv antibody in chronic liver disease in czechoslovakia. Journal of Hepatology 11(SUPPL 2): S114

Sansonno D.; Dammacco F., 1991: Anti c 100 antibody of igm isotype in hepatitis c virus associated chronic liver disease. Gentilini, P And M U Dianzani (Ed ) International Congress Series, No 995 Experimental And Clinical Hepatology; Annual Meeting Of The Italian National Programme on Liver Cirrhosis And Viral Hepatitis, Pisa, Italy, January 9-11, 1992 Xvii+287p Elsevier Science Publishers B V : Amsterdam, Netherlands; (Dist in The Usa And Canada By Elsevier Science Publishing Co , Inc : New York, New York, Usa) Illus 181-188

Barcena R.; Herrero C.; Fernandez Munoz R.; Martin D.A.gila C.; Ulibarrena C.; Pascasio J., 1991: Prevalence and role of antibody to virus c anti vhc in non b non alcoholic chronic liver disease. Journal of Hepatology 13(SUPPL 2): S94

Tsuchiya, K.; Kiyosawa, K.; Imai, H.; Sodeyama, T.; Furuta, S., 1994: Detection of anti-double and anti-single stranded DNA antibodies in chronic liver disease: significance of anti-double stranded DNA antibody in autoimmune hepatitis. Anti-DNA antibodies were determined by an enzyme-linked immunosorbent assay in 116 patients with chronic liver disease consisting of 21 cases of autoimmune hepatitis (AIH), 17 of primary biliary cirrhosis (PBC), and 78 of non-autoimmune-type of ch...

Buzzelli, G.; Alterini, B.; Monaco, L.; Gialdini, D.; Baronti, E.; Matassi, L.; Simondi, P.; Zignego, A.L.; Gentilini, P., 1985: Serum markers of hepatitis B virus and anti-delta antibody in chronic liver disease. Quaderni Sclavo di Diagnostica Clinica E di Laboratorio 21(4): 387-398

Barcena R.; Martin D.A.gila C.; Nash R.; Aviles J.; Aguilera D., 1991: Prevalence and role of antibody to virus c anti hcv in non b chronic liver disease in alcohol abuses. Journal of Hepatology 13(SUPPL 2): S94

Kobayashi, T.; Kaneko, Y.; Sato, J.; Yamada, R.; Harada, T., 1992: Prevalence of anti-GOR antibody production in hepatitis C virus RNA-positive non-A, non-B chronic liver disease. Sera from 41 patients suffering from non-A, non-B (NANB) liver disease were tested for various antibodies and HCV RNA to find relations between hepatitis C virus (HCV) infection and autoantibody production. Anti-HCV (C100-3) antibody was detected...