EurekaMag.com logo
+ Site Statistics
References:
47,893,527
Abstracts:
28,296,643
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Antisense blockade of inducible nitric oxide synthase in glial cells derived from adult SJL mice






Neuroscience Letters 220(2): 89-92

Antisense blockade of inducible nitric oxide synthase in glial cells derived from adult SJL mice

Increasing evidence suggests a correlation between cytokine-induced nitric oxide synthase (iNOS) and demyelination in Multiple sclerosis (MS). Inhibition of iNOS may therefore be a novel therapeutic approach in MS. To test an antisense oligodeoxynucleotide (ODN) knockdown strategy for inhibiting iNOS, we used lipopolysaccharide (LPS) together with gamma-interferon (IFN-gamma) to induce iNOS in adult mouse mixed glial cell cultures. We administered an iNOS-derived antisense phosphorothiorate oligodeoxynucleotide (S-ODN) to block the induction. The antisense ODN treatment resulted in significant inhibition of LPS and IFN-gamma induced iNOS mRNA and protein expression. It also inhibited nitric oxide (NO) and cyclic GMP (cGMP) production in a dose dependent fashion. Sense and random S-oligo had no effect in any of these studies. These data indicate the efficacy and specificity of the antisense oligodeoxynucleotide approach in inhibiting iNOS in glial cells.

Accession: 009770868

PMID: 8981480

DOI: 10.1016/s0304-3940(96)13229-8

Download PDF Full Text: Antisense blockade of inducible nitric oxide synthase in glial cells derived from adult SJL mice



Related references

Ding, M.; Zhang, M.; Wong, J.L.; Voskuhl, R.R.; Ellison, G.W., 1996: Antisense blockade of inducible nitric oxide synthase in glial cells derived from adult SJL mice. Increasing evidence suggests a correlation between cytokine-induced nitric oxide synthase (iNOS) and demyelination in Multiple sclerosis (MS). Inhibition of iNOS may therefore be a novel therapeutic approach in MS. To test an antisense oligodeoxyn...

Thomae, K.R.; Geller, D.A.; Billiar, T.R.; Davies, P.; Pitt, B.R.; Simmons, R.L.; Nakayama, D.K., 1993: Antisense oligodeoxynucleotide to inducible nitric oxide synthase inhibits nitric oxide synthesis in rat pulmonary artery smooth muscle cells in culture. Background. We recently demonstrated the induced expression of the inducible nitric oxide synthase (iNOS) gene in cultured rat pulmonary artery smooth muscle (RPASM) in response to lipopolysaccharide and cytokines, using a complementary DNA probe...

Kitamura, Y.; Furukawa, M.; Matsuoka, Y.; Tooyama, I.; Kimura, H.; Nomura, Y.; Taniguchi, T., 1998: In vitro and in vivo induction of heme oxygenase-1 in rat glial cells: possible involvement of nitric oxide production from inducible nitric oxide synthase. To determine whether heme oxygenase-1 (HO-1) protein is induced by endogenous nitric oxide (NO) in rat glial cultures, we examined the effects of lipopolysaccharide (LPS), interferon-gamma (IFN-gamma), and NO donors such as S-nitroso-N-acetylpenic...

Johns, D.G.; Xu, S.; Cohen, R., 2001: Inducible nitric oxide synthase-derived nitric oxide contributes to peroxynitrite production and attenuates the pressor response in angiotensin II-infused mice. Circulation 104(17 Supplement): II 100, October 23

Nomura, Y.; Kitamura, Y., 1993: Inducible nitric oxide synthase in glial cells. Nitric oxide (NO), a free radical gas, has been suggested to mediate both synaptic plasticity and neuronal death. NO is generated by constitutive and inducible types of NO synthase (cNOS and iNOS, respectively). The neuronal cNOS was recently clon...

O'banion, M.; Dusel, J.C.; Chang, J.W.; Coleman, P.D., 1994: Interleukin-1-beta induction of prostaglandin G/H synthase-2 and inducible nitric oxide synthase in cultured murine glial cells. Society for Neuroscience Abstracts 20(1-2): 1496

ZhuangYing; ZhangYaPing; MaSiYuan, 2008: Effects of Fabin immunoglobulin Y against lipopolysaccharide on expression of nitric oxide, inducible nitric oxide synthase and malondialdehyde during the early stage in severely burned gut-derived endotoximia mice. A study was conducted to explore a new way of preventing burn sepsis, and to investigate the effect of the Fab' against endotoxins on nitric oxide (NO), inducible nitric oxide synthase (iNOS) and malondialdehyde (MDA) during the early stage i...

Kitamura, Y.; Nomura, Y., 1994: Inducible type of nitric oxide synthase in rat glial cells. Takagi, H [Editor], Toda, N [Editor], Hawkins, R D [Editor] Taniguchi Symposia on Brain Sciences; Nitric oxide: Roles in neuronal communication and neurotoxicity 41-51

Zhang, M.; Wong, J.L.; Voskuhl, R.R.; Ellison, G.W.; Ding, M., 1996: Inhibition of experimental autoimmune encephalomyelitis in mice by antisense knockout of inducible nitric oxide synthase. Society for Neuroscience Abstracts 22(1-3): 2136

Tanjoh, K.; Tomita, R.; Hayashi, N., 2000: Antisense oligodeoxynucleotides to human inducible nitric oxide synthase selectively inhibit induced nitric oxide production by human vascular endothelial cells: an experimental study. To find out whether the antisense oligodeoxynucleotide designed against the complementary DNA to human inducible NO synthase (iNOS) would block the translation from iNOS mRNA to NO in human vascular endothelial cells. Prospective controlled study....