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Chromophore distortions in the bacteriorhodopsin photocycle: evolution of the H-C14-C15-H dihedral angle measured by solid-state NMR


Chromophore distortions in the bacteriorhodopsin photocycle: evolution of the H-C14-C15-H dihedral angle measured by solid-state NMR



Biochemistry 41(2): 431-438



ISSN/ISBN: 0006-2960

PMID: 11781081

DOI: 10.1021/bi011529r

In recent years, structural information about bacteriorhodopsin has grown substantially with the publication of several crystal structures. However, precise measurements of the chromophore conformation in the various photocycle states are still lacking. This information is critical because twists about the chromophore backbone chain can influence the Schiff base nitrogen position, orientation, and proton affinity. Here, we focus on the C14-C15 bond, using solid-state nuclear magnetic resonance spectroscopy to measure the H-C14-C15-H dihedral angle. In the resting state (bR568), we obtain an angle of 164 [plus or minus] 4[degree], indicating a 16[degree] distortion from a planar all-trans chromophore. The dihedral angle is found to decrease to 147 [plus or minus] 10[degree] in the early M intermediate (Mo) and to 150 [plus or minus] 4[degree] in the late M intermediate (Mn). These results demonstrate changes in the chromophore conformation undetected by recent X-ray diffraction studies. Reprinted by permission of the publisher.

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Accession: 009793443

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