+ Site Statistics
References:
54,258,434
Abstracts:
29,560,870
PMIDs:
28,072,757
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Debrisoquine polymorphism: Novel CYP2D6 gene Bam HI restriction fragment length polymorphism in the Ngawbe Guaymi Indian of Panama



Debrisoquine polymorphism: Novel CYP2D6 gene Bam HI restriction fragment length polymorphism in the Ngawbe Guaymi Indian of Panama



Pharmacogenetics 1(3): 136-142



Markedly decreased cytochrome P450-mediated metabolism of debrisoquine, sparteine, and more than two dozen additional commonly prescribed drugs is an autosomal recessive trait that has been associated with several RFLP patterns involving the CYP2D6 gene. In Caucasians there are at least six variant alleles known to be correlated with the 'poor metabolizer' (PM) phenotype. We examined debrisoquine and sparteine metabolism and CYP2D6 RFLP patterns in 22 Ngawbe Guaymi Indians of Panama. We studied a two-generation family, a three-generation family, and three other unrelated PM individuals. Digestion of all 22 DNA samples with Xba I or Hind III did not produce the same varying CYP2D6 RFLP patterns as those commonly seen in at least two-thirds of all Northern European Caucasians and Chinese so far screened. In contrast, we found a single heretofore undescribed Bam HI polymorphism that was correlated with the PM phenotype among all Ngawbe Guaymi individuals examined. It is possible that this novel RFLP might represent a recent founder effect that has occurred in this unadmixed Amerindian tribe within the past 20 000-30 000 years.

(PDF emailed within 1 workday: $29.90)

Accession: 009810574

Download citation: RISBibTeXText

PMID: 1688244


Related references

An observation on the ethnic uniqueness of the debrisoquine and sparteine antimodes: a study in the Ngawbé Guaymí Amerindians of Panamá. British Journal of Clinical Pharmacology 28(4): 493-494, 1989

Genetic polymorphism of debrisoquine oxidation: restriction fragment analysis and allele-specific amplification of mutant alleles of CYP2D6. Methods in Enzymology 206: 173-183, 1991

Evaluation of PCR-restriction profile analysis and IS2404 restriction fragment length polymorphism and amplified fragment length polymorphism fingerprinting for identification and typing of Mycobacterium ulcerans and M. marinum. Journal of Clinical Microbiology 39(9): 3272-3278, 2001

Detection of CYP2D6*3 and 2D6*4 allelic variants by PCR-restriction fragment length polymorphism. Clinical Chemistry and Laboratory Medicine 36(8): 655-658, 1998

Evolutionary pharmacogenetics of CYP2D6 in Ngawbe Guaymi of Panama: allele-specific PCR detection of the CYP2D6B allele and RFLP analysis. Pharmacogenetics 3(5): 231-238, 1993

Sequence polymorphism and PCR-restriction fragment length polymorphism analysis of the flagellin gene of Burkholderia pseudomallei. Journal of Clinical Microbiology 48(4): 1465-1467, 2010

Restriction fragment length polymorphism (RFLP) analysis of buffalo thyroglobulin gene 1. Polymorphism in the left (5') region. Indian Journal of Veterinary Research 5(2): 19-27, 1996

Population genetics of the yellow fever mosquito in Trinidad Comparisons of amplified fragment length polymorphism and restriction fragment length polymorphism markers. Molecular Ecology 8(6): 951-963, June, 1999

Amplified-fragment length polymorphism as a complement to IS6110-based restriction fragment length polymorphism analysis for molecular typing of Mycobacterium tuberculosis. Journal of Clinical Microbiology 41(10): 4820-4822, 2003

Assessing genetic variability among Brazilian strains of Xanthomonas axonopodis pv. manihotis through restriction fragment length polymorphism and amplified fragment length polymorphism analyses. Canadian Journal of Microbiology 45(9): 754-763, 1999

Comparison of 23S polymerase chain reaction-restriction fragment length polymorphism and amplified fragment length polymorphism techniques as typing systems for thermophilic campylobacters. FEMS Microbiology Letters 211(1): 97-103, 2002

Differentiation of Candida glabrata, C. nivariensis and C. bracarensis based on fragment length polymorphism of ITS1 and ITS2 and restriction fragment length polymorphism of ITS and D1/D2 regions in rDNA. European Journal of Clinical Microbiology & Infectious Diseases 30(11): 1409-1416, 2014

Population genetics of the yellow fever mosquito in Trinidad: comparisons of amplified fragment length polymorphism (AFLP) and restriction fragment length polymorphism (RFLP) markers. Molecular Ecology. June; 86: 951-963, 1999