+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Demonstration of differential effects of cytokines on mast cells derived from murine bone marrow and peripheral blood mononuclear cells



Demonstration of differential effects of cytokines on mast cells derived from murine bone marrow and peripheral blood mononuclear cells



Experimental Hematology 22(12): 1147-1155



Mouse bone marrow (BM) was cultured in the presence of recombinant mouse (rm) interleukin-3 (IL-3), rmIL-4, rmIL-5, rmIL-7, purified mouse (m) IL-9, rmIL-10, recombinant human (rh) macrophage-colony-stimulating factors (M-CSF), rm granulocyte-macrophage colony-stimulating factors (GM-CSF), rm stem cell factor (SCF), rh interferon-alpha (IFN-alpha), rmIFN-gamma, and mNGF to determine which cytokine would give rise to mast cells in murine BM cultures. From a starting population of 1 times 10-7 cells, 1.55 times 10-7 mast cells developed within 14 days in cultures supplemented by rmIL-3. No mast cells were seen at day 14 when any of the other cytokines were present alone, except for rmSCF, which supported the growth of lt 0.01% of mast cells observed in IL-3-dependent BM cultures. When rmIL-4, -5, -7, -10, mIL-9, rhM-CSF, rmGM-CSF, rmSCF, rhIFN-alpha, -gamma, or mNGF were added to BM cultures in the presence of rmIL-3, mast cell growth increased 200% with the addition of rmSCF, and 10% when rmIL-4 or IL-9 was added. However, the addition of rhM-CSF, rmGM-CSF, rmIFN-gamma, and mNGF decreased the number of mast cells. Mast cell number, as determined by metachromatic stains, generally approximated the number of Fc-epsilon-RI+ cells as assessed by FACS analysis. Among the cytokines, only rmIL-4 and rmSCF were able to support the survival of mast cell progenitors in the absence of obvious mast cell proliferation, similarly to rmIL-3. Only rmSCF alone, or in combination with rmIL-3 or -4, supported the growth of mast cells from mouse peripheral blood mononuclear cells (PBMC) where the number of mast cell precursors was about 90 per 10-6 PBMC. With time, mouse BM cells cultured in rmIL-3 became more responsive to rmSCF. Taken together, these data demonstrate that IL-3 is a major early mast cell growth factor, that mast cells become more dependent on SCF with time, and that the effects of IL-3 and SCF are upregulated (IL-4) or downregulated (M-CSF, GM-CSF, IFN-gamma) by both growth factors and proinflammatory cytokines.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 009812081

Download citation: RISBibTeXText

PMID: 7523167


Related references

TLR3-, TLR7-, and TLR9-mediated production of proinflammatory cytokines and chemokines from murine connective tissue type skin-derived mast cells but not from bone marrow-derived mast cells. Journal of Immunology 173(1): 531-541, 2004

Expression of Thymosin beta4 in Murine Bone Marrow Endothelial Cells, HL-60 Cells and Human Peripheral Blood Mononuclear Cells. Zhongguo Shi Yan Xue Ye Xue Za Zhi 9(1): 10-13, 2001

Peripheral blood mononuclear cells from recipients of human bone marrow allografts suppress mitogen induced proliferation of marrow donor peripheral blood mononuclear cells. Transplantation Proceedings: 270-272, 1986

Generation of dendritic cells from human bone marrow mononuclear cells: advantages for clinical application in comparison to peripheral blood monocyte derived cells. International Journal of Oncology 20(2): 247-253, 2002

Yolk sac-derived murine mast cells exhibit lower levels of telomerase activity than bone marrow derived mast cells. FASEB Journal 12(5): A895, 1998

Growth of human mast cells from bone marrow and peripheral blood-derived CD34+ pluripotent progenitor cells. Methods in Molecular Biology 315: 105-112, 2006

Growth of human mast cells from bone marrow and peripheral blood-derived CD34(+) pluripotent hematopoietic cells. Methods in Molecular Biology 1220: 155-162, 2015

Effects of TGF-β and b-FGF on the potential of peripheral blood-borne stem cells and bone marrow-derived stem cells in wound healing in a murine model. Inflammation 35(1): 138-142, 2012

Adherent cell layers formed by G-CSF mobilized peripheral blood mononuclear cells support bone marrow and peripheral blood derived LTC-IC. Onkologie 18(Suppl. 2): 169, 1995

Induction of Ym1/2 in mouse bone marrow-derived mast cells by IL-4 and identification of Ym1/2 in connective tissue type-like mast cells derived from bone marrow cells cultured with IL-4 and stem cell factor. Immunology and Cell Biology 83(5): 468-474, 2005

The role of monocytes in the pokeweed mitogen response of human peripheral blood mononuclear cells separate signals for lymphocyte proliferation and differentiation of bone marrow derived cells into immuno globulin secreting cells. Federation Proceedings 39(3): ABSTRACT 4122, 1980

Mast cell heterogeneity. Differential synthesis and expression of glycosphingolipids by mouse serosal mast cells as compared to IL-3-dependent bone marrow culture-derived mast cells before or after coculture with 3T3 fibroblasts. Journal of Immunology 145(5): 1463-1468, 1990

Effects of mast cell costimulatory activity mca a t h2 cell derived lymphokine identical with il10 on the development of murine bone marrow derived mast cells bmmc. Immunobiology 183(3-4): 228, 1991

Induction of differentiation of human mast cells from bone marrow and peripheral blood mononuclear cells by recombinant human stem cell factor/kit-ligand in long-term culture. Blood 80(9): 2237-2245, 1992

Toxicity of coumarin (1,2-benzopyrone) on human peripheral blood mononuclear cells and human and murine bone marrow progenitor stem cells. Journal of Biological Response Modifiers 8(2): 116-121, 1989