+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Differential display RT-PCR analysis of enterovirus-71-infected rhabdomyosarcoma cells reveals mRNA expression responses of multiple human genes with known and novel functions



Differential display RT-PCR analysis of enterovirus-71-infected rhabdomyosarcoma cells reveals mRNA expression responses of multiple human genes with known and novel functions



Virology 295(1): 147-159



In order to better understand cellular responses to viral infection at the transcriptional level, we employed differential display RT-PCR to analyze mRNAs from RD rhabdomyosarcoma cells following infection with a neurovirulent enterovirus 71 (EV71) strain, compared with mRNAs from uninfected cells. Of 250 expressed sequence tags (ESTs) isolated, sequenced, and identified, all were of cellular origin except 1 that was of viral origin. Of these, 156 were individual distinctive clones, comprising 45 mRNAs showing unaltered expression and 111 mRNAs exhibiting upregulation or downregulation. Of the 45 uniformly expressed mRNAs, 14 represented unknown genes. Of the 111 differentially expressed mRNAs, 63 did not match any known genes. Forty-eight of the 111 mRNAs modified by EV71 infection matched known genes, including those encoding components of cell cycle, cytoskeleton, and cell death mediators; protein degradation mediators; mitochondrial-related proteins; components of protein translation and modification; and cellular transport proteins. The altered expression profiles of representative genes were authenticated by semiquantitative RT-PCR and real-time RT-PCR. We also identified a novel alternatively spliced transcript of TRIP7 thyroid receptor interactor protein; the putative human homolog of murine mc7 mRNA predominantly expressed in the brain; and a novel mRNA similar to that encoding vacuolar protein 8 involved in protein targeting. These results underscore the applicability of the mRNA differential display technique for elucidating the expression profiles of known and even novel genes in response to cellular infection with pathogenic viruses.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 009817608

Download citation: RISBibTeXText

PMID: 12033773

DOI: 10.1006/viro.2002.1353


Related references

Differential display RT-PCR analysis of ECV304 endothelial-like cells infected with dengue virus type 2 reveals messenger RNA expression profiles of multiple human genes involved in known and novel roles. Journal of Medical Virology 72(4): 597-609, 2004

Differential mRNA display analysis of gene expression in Cryptosporidium parvum-infected HCT-8 cells. Journal of Eukaryotic Microbiology 43(5): 80s-81s, 1996

Differential display of human marrow stromal cells reveals unique mRNA expression patterns in response to dexamethasone. Journal of Cellular Biochemistry 76(2): 231-243, 1999

Proteomic analysis of human brain microvascular endothelial cells reveals differential protein expression in response to enterovirus 71 infection. Biomed Research International 2015: 864169, 2015

Search for genes involved in UV-resistance in human cells by mRNA differential display: increased transcriptional expression of nucleophosmin and T-plastin genes in association with the resistance. Biochemical and Biophysical Research Communications 248(3): 597-602, 1998

Next-Generation Sequencing Analysis Reveals Differential Expression Profiles of MiRNA-mRNA Target Pairs in KSHV-Infected Cells. Plos one 10(5): E0126439, 2015

Searching for excystment-regulated genes in Sterkiella histriomuscorum (Ciliophora, Oxytrichidae): a mRNA differential display analysis of gene expression in excysting cells. Journal of Eukaryotic Microbiology 48(3): 382-390, 2001

Searching for excystment-regulated genes in Sterkiella histriomuscorum (Ciliophora, Oxytrichidae): a mRNA differential display analysis of gene expression in excysting cells. Journal of Eukaryotic Microbiology 48(3): 2-90, 2001

Searching for excystment-regulated genes in Sterkiella histriomuscorum Ciliophora, Oxytrichidae a mRNA differential display analysis of gene expression in excysting cells. Journal of Eukaryotic Microbiology: 382-390, 2001

Molecular profiling of transformed and metastatic murine squamous carcinoma cells by differential display and cDNA microarray reveals altered expression of multiple genes related to growth, apoptosis, angiogenesis, and the NF-kappaB signal pathway. Cancer Research 61(12): 4797-4808, 2001

Differential gene expressions of the MAPK signaling pathway in enterovirus 71-infected rhabdomyosarcoma cells. Brazilian Journal of Infectious Diseases 17(4): 410-417, 2014

Differential display analysis reveals the expression of glutathione S-transferase and novel genes through an ITAM-containing receptor in ascidian immunocytes. Immunogenetics y; 57(6): 444-452, 2005

Characterization of Critical Functions of Long Non-Coding RNAs and mRNAs in Rhabdomyosarcoma Cells and Mouse Skeletal Muscle Infected by Enterovirus 71 Using RNA-Seq. Viruses 10(10):, 2018

Identification of glucose-regulated genes in human mesangial cells by mRNA differential display. Biochemical & Biophysical Research Communications. 238(1): 179-184, 1997

Search for gravity-responsive genes by PCR-based mRNA differential display in human cells. Journal of Gravitational Physiology 7(2): P75-P76, 2000