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Dimerization of Thermomonospora fusca b-1,4-endoglucanase E2



Dimerization of Thermomonospora fusca b-1,4-endoglucanase E2



Biochemistry (American Chemical Society) 32: 46-50



Unboiled Thermomonospora fusca endoglucanase E2 electrophoresed on SDS-polyacrylamide gels migrated in the range of 80-90 kDa, but when boiled it migrated in the 40-42-kDa range. Sedimentation equilibrium centrifugation as well as chemical cross-linking experiments confirmed that E2 is a dimer. The dimer was reversibly dissociated at low pH. The E2 dimer was stable up to 70 MoC, but began to dissociate at this temperature after a 30-60-min incubation. A nondimerizing mutant was obtained using region-specific chemical mutagenesis. DNA sequencing of this mutant revealed a single base change that substituted Gly for Glu-263. Chemical modification of carboxylic acid residues in E2 disrupted the dimer interaction. Copyright 1993, American Chemical Society.

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Accession: 009818766

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