Escherichia coli tRNA (Asp) recognition mechanism differing from that of the yeast system
Nameki, N.; Tamura, K.; Himeno, H.; Asahara, H.; Hasegawa, T.; Shimizu, M.
Biochemical and Biophysical Research Communications 189(2): 856-862
ISSN/ISBN: 0006-291X PMID: 1472058 DOI: 10.1016/0006-291x(92)92282-3
Various tRNA transcripts were constructed to study the identity elements of Escherichia coli tRNA-Asp. Base substitutions from G-34 to U-34 at the first position of the anticodon, and from U-35 to A-35 at the second, severely impaired the aspartate charging activity. The activity was also decreased, but in a more moderate fashion, by base changes at G-2-G-71, C-36 and C-38. Identity nucleotides of tRNA-Asp are distributed in a different fashion between E. coli and yeast, which occur at the second base pair of the acceptor stem, G-10-U-25 base pair in the D-stem and 3' half of the anticodon loop.