High-performance liquid chromatographic determination of human serum albumin in plasma and urine by post-column fluorescence enhancement detection using 8-anilino-1-naphthalenesulfonic acid
Ishida, J.; Abe, K.; Nakamura, M.; Yamaguchi, M.
Biological and Pharmaceutical Bulletin 19(11): 1391-1395
ISSN/ISBN: 0918-6158 PMID: 8951151 DOI: 10.1248/bpb.19.1391
A method for the quantitative determination of human serum albumin in plasma and urine by post-column high-performance liquid chromatography with fluorescence detection is presented. Human serum albumin in plasma and urine is separated by gel-permeation column chromatography, which is followed by fluorescence detection. The detection uses the fluorescence enhancement observed when 8-anilino-1-naphthalenesulfonic acid binds to human serum albumin. The method is rapid to perform and is highly sensitive (detection limit is 2.5 ng/injection volume at a signal-to-noise ratio of three).