Section 10
Chapter 9,880

In vivo chromatin remodeling by yeast ISWi homologs Isw1p and Isw2p

Kent, N.A.; Karabetsou, N.; Politis, P.K.; Mellor, J.

Genes and Development 15(5): 619-626


ISSN/ISBN: 0890-9369
PMID: 11238381
DOI: 10.1101/gad.190301
Accession: 009879122

Isw1p and Isw2p are budding yeast homologs of the Drosophila ISWI chromatin-remodeling ATPase. Using indirect-end-label and chromatin immunoprecipitation analysis, we show both independent and cooperative Isw1p- and Isw2p-mediated positioning of short nucleosome arrays in gene-regulatory elements at a variety of transcription units in vivo. We present evidence that both yeast ISWI complexes regulate developmental responses to starvation and that for Isw2p, recruitment by different DNA-binding proteins controls meiosis and haploid invasive growth.

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