EurekaMag.com logo
+ Site Statistics
References:
53,869,633
Abstracts:
29,686,251
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on LinkedInFollow on LinkedIn

+ Translate

In vivo proliferation and cell cycle kinetics of long-term self-renewing hematopoietic stem cells



In vivo proliferation and cell cycle kinetics of long-term self-renewing hematopoietic stem cells



Proceedings of the National Academy of Sciences of the United States of America 96(6): 20-5



The in vivo proliferation and cell cycle kinetics of long-term self-renewing hematopoietic stem cells (LT-HSC) were investigated. Incorporation of bromodeoxyuridine (BrdUrd) label was used to measure the rate at which different cohorts of LT-HSC entered the cell cycle over time. At any one time, about 5 percent of the cells were in S/G2/M phases and 20 percent were in G1 phase. By 6 days, 30 days, 6 months, BrdUrd was incorporated in about 50 percent, over 90 percent, and 99 percent of cells, respectively. Calculations revealed that about 8 percent of LT-HSC asynchronously entered the cell cycle each day. Although about 75 percent of the cells were quiescent in G0 at any given time, all HSC were recruited into the cycle regularly so that 99 percent of the cells divided on average every 57 days. A model for LT-HSC cell cycle regulation is proposed.

(PDF emailed within 0-6 h: $19.90)

Accession: 009879320

Download citation: RISBibTeXText

PMID: 10077647

DOI: 10.2307/47499



Related references

Systemic Virus Infections Differentially Modulate Cell Cycle State and Functionality of Long-Term Hematopoietic Stem Cells In Vivo. Cell Reports 19(11): 2345-2356, 2018

Proliferation of totipotent hematopoietic stem cells in vitro with retention of long-term competitive in vivo reconstituting ability. Proceedings of the National Academy of Sciences of the United States of America 89(5): 1968-1972, 1992

High-resolution tracking of cell division suggests similar cell cycle kinetics of hematopoietic stem cells stimulated in vitro and in vivo. Blood 95(3): 855-862, 2000

High resolution tracking of cell divisions demonstrates similar cell cycle entry kinetics of hematopoietic stem cells stimulated in vitro and in vivo. Experimental Hematology (Charlottesville) 26(8): 814, 1998

Hematopoietic stem cell tracking in vivo: a comparison of short-term and long-term repopulating cells. Blood 93(6): 1916-1921, 1999

Long-term repopulation of irradiated mice with limiting numbers of purified hematopoietic stem cells: in vivo expansion of stem cell phenotype but not function. Blood 85(4): 1006-1016, 1995

Cell-intrinsic in vivo requirement for the E47-p21 pathway in long-term hematopoietic stem cells. Journal of Immunology 192(1): 160-168, 2014

Proliferation kinetics and length of cell cycle of individual long-term culture initiating cells. Experimental Hematology (Charlottesville) 26(8): 684, 1998

Cell cycle and proliferation kinetics of a hematopoietic stem cell expansion culture. Annals of Hematology 77(SUPPL 2): S28, 1998

Long-term in vivo provision of antigen-specific T cell immunity by programming hematopoietic stem cells. Proceedings of the National Academy of Sciences of the United States of America 102(12): 4518-4523, 2005

FT3 ligand and stem cell factor support the ex vivo expansion of primitive human hematopoietic cells Response of long-term culture initiating cells and CD34+ CD38-d-i-m cells. Blood 84(10 SUPPL 1): 368A, 1994

Hypoxia mediates low cell-cycle activity and increases the proportion of long-term-reconstituting hematopoietic stem cells during in vitro culture. Experimental Hematology 38(4): 301-310.E2, 2010

Identification of factors promoting ex vivo maintenance of mouse hematopoietic stem cells by long-term single-cell quantification. Blood 128(9): 1181-1192, 2017

Treatment of a mouse model of spinal cord injury by transplantation of human induced pluripotent stem cell-derived long-term self-renewing neuroepithelial-like stem cells. Stem Cells 30(6): 1163-1173, 2012

Rapamycin enhances long-term hematopoietic reconstitution of ex vivo expanded mouse hematopoietic stem cells by inhibiting senescence. Transplantation 97(1): 20-29, 2014