+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Kex2 family endoprotease furin is expressed specifically in pit-region parietal cells of the rat gastric mucosa



Kex2 family endoprotease furin is expressed specifically in pit-region parietal cells of the rat gastric mucosa



American Journal of Physiology 277(1 Pt 1): G183-G190



The proprotein-processing endoprotease furin is localized in the gastric epithelial cells of the pit region in the rat gastric gland. The gastric pit is composed of several cell types, including gastric surface mucosal (GSM) cells and parietal cells. Furin converts many growth- or differentiation-related proproteins to their active forms. We examined identification of furin-positive cells by immunostaining of rat gastric mucosa and regulators of the furin expression by measuring the furin promoter activity by luciferase assay. Furin-positive cells were stained for H+-K+-ATPase, indicating that they are parietal cells. Furin-positive parietal cells were not stained for transforming growth factor-a (TGF-a) but were surrounded by TGF-a-positive GSM cells. In contrast, parietal cells below the proliferative zone were positive for TGF-a but not for furin. Furin-positive parietal cells expressed a high level of epidermal growth factor receptor (EGFR). TGF-a stimulated the furin promoter activity highly in a mouse GSM cell line GSM06. Thus we suggest that the parietal cells of the pit region have furin-mediated functions that can be stimulated by EGFR signaling. Reprinted by permission of the publisher.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 009893320

Download citation: RISBibTeXText

PMID: 10409166


Related references

Kex2 family endoprotease furin is expressed specifically in pit-region parietal cells of the rat gastric mucosa. American Journal of Physiology. Gastrointestinal and Liver Physiology 277(1): G183-G190, 2018

Limulus kexin: A new type of Kex2-like endoprotease specifically expressed in hemocytes of the horseshoe crab. FEBS Letters 386(2-3): 201-204, 1996

Purification and characterization of furin, a Kex2-like processing endoprotease, produced in Chinese hamster ovary cells. Journal of Biological Chemistry 267(23): 16094-9, 1992

Isolation of a cDNA encoding a Kex2-like endoprotease with homology to furin from the nematode Caenorhabditis elegans. Dna and Cell Biology 16(5): 663-669, 1997

Molecular and enzymatic properties of furin, a Kex2-like endoprotease involved in precursor cleavage at Arg-X-Lys/Arg-Arg sites. Journal of Biochemistry 111(3): 296-301, 1992

Cannabinoid CB1 receptors are expressed by parietal cells of the human gastric mucosa. Journal of Histochemistry and Cytochemistry 56(5): 511-516, 2008

Drebrin E2 is differentially expressed and phosphorylated in parietal cells in the gastric mucosa. American Journal of Physiology. Gastrointestinal and Liver Physiology 289(2): G320-G331, 2005

Proprotein-processing endoprotease furin controls the growth and differentiation of gastric surface mucous cells. Journal of Clinical Investigation 99(8): 1842-1851, 1997

Mutations in the yeast KEX2 gene cause a Vma(-)-like phenotype: a possible role for the Kex2 endoprotease in vacuolar acidification. Molecular and Cellular Biology 18(3): 1534-1543, 1998

A unique Kex2-like endoprotease from Drosophila melanogaster is expressed in the central nervous system during early embryogenesis. Journal of Neuroscience 12(3): 705-717, 1992

The pro-region of the Kex2 endoprotease of Saccharomyces cerevisiae is removed by self-processing. Febs Letters 299(3): 283-286, 1992

Identification of an isoform with an extremely large Cys-rich region of PC6, a Kex2-like processing endoprotease. Febs (federation Of European Biochemical Societies) Letters. 327(2): 165-171, 1993

Specific modulation of Kex2/furin family proteases by potassium. Journal of Biological Chemistry 277(20): 17531-7, 2002

Activation of pro-region chimeras of furin endoprotease. Journal of Cellular Biochemistry Supplement 0(19B): 244, 1995

Changes in the gastric mucosa in experimental uremia. Behavior of the parietal cells in the gastric mucosa of uremic mice. An autoradiographic study. Zeitschrift für Gastroenterologie 9(9): 639-645, 1971