EurekaMag
+ Most Popular
Cunninghamia lanceolata plantations in China
Mammalian lairs in paleo ecological studies and palynology
Studies on technological possibilities in utilization of anhydrous milk fat for production of recombined butter-like products
Should right-sided fibroelastomas be operated upon?
Large esophageal lipoma
Apoptosis in the mammalian thymus during normal histogenesis and under various in vitro and in vivo experimental conditions
Poissons characoides nouveaux ou non signales de l'Ilha do Bananal, Bresil
Desensitizing efficacy of Colgate Sensitive Maximum Strength and Fresh Mint Sensodyne dentifrices
Administration of fluid by subcutaneous infusion: revival of a forgotten method
Tundra mosquito control - an impossible dream?
Schizophrenia for primary care providers: how to contribute to the care of a vulnerable patient population
Geochemical pattern analysis; method of describing the Southeastern limestone regional aquifer system
Incidence of low birth weights in a hospital of Mexico City
Tabanidae
Graded management intensity of grassland systems for enhancing floristic diversity
Microbiology and biochemistry of cheese and fermented milk
The ember tetra: a new pygmy characid tetra from the Rio das Mortes, Brazil, Hyphessobrycon amandae sp. n. (Pisces, Characoidei)
Risk factors of contrast-induced nephropathy in patients after coronary artery intervention
Renovation of onsite domestic wastewater in a poorly drained soil
Observations of the propagation velocity and formation mechanism of burst fractures caused by gunshot
Systolic blood pressure in a population of infants in the first year of life: the Brompton study
Haematological studies in rats fed with metanil yellow
Studies on pasteurellosis. I. A new species of Pasteurella encountered in chronic fowl cholera
Dormancy breaking and germination of Acacia salicina Lindl. seeds
therapy of lupus nephritis. a two-year prospective study

Melanoma loss-of-function mutants in Xiphophorus caused by Xmrk-oncogene deletion and gene disruption by a transposable element


Melanoma loss-of-function mutants in Xiphophorus caused by Xmrk-oncogene deletion and gene disruption by a transposable element



Genetics 153(3): 1385-1394



ISSN/ISBN: 0016-6731

PMID: 10545466

The overexpression of the Xmrk oncogene (ONC-Xmrk) in pigment cells of certain Xiphophorus hybrids has been found to be the primary change that results in the formation of malignant melanoma. Spontaneous mutant stocks have been isolated that have lost the ability to induce tumor formation when crossed with Xiphophorus helleri. Two of these loss-of-function mutants were analyzed for genetic defects in ONC-Xmrk's. In the lof-1 mutant a novel transposable element, TX-1, has jumped into ONC-Xmrk, leading to a disruption of the gene and a truncated protein product lacking the carboxyterminal domain of the receptor tyrosine kinase. TX-1 is obviously an active LTR-containing retrotransposon in Xiphophorus that was not found in other fish species outside the family Poeciliidae. Surprisingly, it does not encode any protein, suggesting the existence of a helper function for this retroelement. In the lof-2 mutant the entire ONC-Xmrk gene was found to be deleted. These data show that ONC-Xmrk is indeed the tumor-inducing gene of Xiphophorus and thus the critical constituent of the tumor (Tu) locus. Reprinted by permission of the publisher.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 009906823

Download citation: RISBibTeXText

Related references

Characterization of the Xmrk proto-oncogene structure and function in relation to melanoma formation in Xiphophorus. Biological Chemistry Hoppe-Seyler 375(SPEC Suppl 1): S40, 1994

Hypomethylation of the Xmrk oncogene promoter in melanoma cells of Xiphophorus. Biological Chemistry 378(12): 1457-1466, 1997

Transcriptional activation of the melanoma inducing Xmrk oncogene in Xiphophorus. Oncogene 6(1): 73-80, 1991

The macromelanophore locus and the melanoma oncogene Xmrk are separate genetic entities in the genome of Xiphophorus. Genetics 149(4): 1909-1920, 1998

Intragenic sex-chromosomal crossovers of Xmrk oncogene alleles affect pigment pattern formation and the severity of melanoma in Xiphophorus. Genetics 151(2): 773-783, 1999

Activation of transcription of the melanoma inducing Xmrk oncogene by a GC box element. Journal of Biological Chemistry 272(1): 131-137, 1997

Differences in transcription and promoters of Xmrk-1 and Xmrk-2 genes suggest a role for Xmrk-2 in pigment pattern development in the platyfish, Xiphophorus maculatus. Cell Growth and Differentiation: the Molecular Biology Journal of the American Association for Cancer Research 5(6): 575-583, 1994

Differences in transcription and promotes of Xmrk-1 and Xmrk-2 genes suggest a role for Xmrk-2 in pigment pattern development in the platyfish, Xiphophorus maculatus. Cell Growth and Differentiation 5(6): 575-583, 1994

Activation of the Xmrk proto-oncogene of Xiphophorus by overexpression and mutational alterations. Oncogene 16(13): 1681-1690, 1998

The Xmrk oncogene can escape nonfunctionalization in a highly unstable subtelomeric region of the genome of the fish Xiphophorus. Genomics 82(4): 470-479, 2003

The melanoma inducing growth factor receptor xmrk of xiphophorus. Journal of Cancer Research and Clinical Oncology 116(Suppl Part 2): 931, 1990

Constitutive activation and specific signaling of the Xmrk receptor in Xiphophorus melanoma. Marine Biotechnology (New York) 4(3): 208-217, 2002

The Xmrk receptor tyrosine kinase is activated in Xiphophorus malignant melanoma. Embo Journal 11(11): 4239-4246, 1992

Expression regulation triggers oncogenicity of xmrk alleles in the Xiphophorus melanoma system. Comparative Biochemistry and Physiology. Toxicology and Pharmacology: Cbp 155(1): 71-80, 2012

Receptor tyrosine kinase Xmrk mediates proliferation in Xiphophorus melanoma cells. International Journal of Cancer 76(3): 437-442, 1998