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Pretreatment of wood by UCT-solvent for the enzymatic hydrolysis



Pretreatment of wood by UCT-solvent for the enzymatic hydrolysis



Applied Biochemistry & Biotechnology 37(2): 165-176



UCT-solvent pretreatment was carried out on woods (beech and akamatsu (pine)) for the enzymatic hydrolysis, in which pretreatment the ground woods were autoclaved with a mixture of water and cyclohexanol (37.5% vol% cyclohexanol) having upper critical temperature (UCT: 184 degree C) on the mutual solubility curve (named as UCT-solvent). Ninety-five and 92% of Klason lignin were removed from beech and akamatsu, respectively, wheras when the woods were autoclaved with water instead of UCT-solvent, only 43 and 18% of Klason lignin was removed from them, respectively. The excellent ability of UCT-solvent for the removal of klason lignon is owing to that the solvent disturbs re-coupling between the degradation products. The enzymatic hydrolysis of wood was much improved by UCT-solvent pretreatment: the hydrolytic reactivity of akamatsu was enhanced by 2.8 times comparing with when akamatus was pretreated with water instead of UCT-solvent.

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Accession: 009946439

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DOI: 10.1007/bf02921668


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