Protein kinase C activation upregulates intercellular adhesion of a-catenin-negative human colon cancer cell variants via induction of desmosomes

Van Hengel, J.; Gohon, L.; Bruyneel, E.

The Journal of Cell Biology 137(5): 03-16


Accession: 009949906

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Several invasive human colon carcinoma cell lines lacking a-catenin were studied. Increased invasiveness correlated with a solitary and rounded morphotype, which acquired a more normal epithelial phenotype on transfection with an a-catenin expression plasmid and on treatment with the protein kinase C activator 12-O-tetradecanoyl-phorbol-13-acetate (TPA). Cells established intercellular junctions within 30 min after TPA addition. This effect was not associated with a-catenin induction and entailed only minor changes in E-cadherin staining. In contrast, desmosomal and tight junctional proteins were significantly rearranged, and typical desmosomal plaques were apparent. The TPA-restored cell-cell adhesion was E-cadherin dependent, and the addition of an antibody against the extracellular part of desmoglein-2 blocked the TPA effect. Anti-E-cadherin and anti-desmoglein antibodies synergistically inhibited the TPA effect.