Recognition of a special membrane antigen of squamous cell carcinoma in rats with a monoclonal antibody UB23
Li, X.; Fujikura, Y.; Wang, Y.H.; Sawada, T.; Tokuda, N.; Lovely, R.S.; Hayatsu, Y.; Fukumoto, T.; Shinozaki, F.
Tumour Biology the Journal of the International Society for Oncodevelopmental Biology and Medicine 18(6): 350-355
ISSN/ISBN: 1010-4283 PMID: 9372868 DOI: 10.1159/000218049
This article describes the recognition of a special membrane antigen of the rat squamous cell carcinoma (SCC) by a monoclonal antibody (mAb), UB23, and the characterization of the UB23 antigen expression in the implanted primary and metastatic SCC in rat models. The mAb UB23 was raised against the FF6 tumor, a well-differentiated rat SCC, and it recognized the 120- to 130-kD cell surface antigen in FF6 tumor cells. The UB23 antigen was found in frequently observed small 'basal' cells but not in keratinocytes, and an increased expression was seen in the cells at the interface with peritumoral stroma in both the implanted primary FF6 tumors and metastases. These results indicated that the UB23 antigen is closely related with the cell differentiation and invasion of FF6 cells, and could be useful for analyzing the mechanism of differentiation, invasion and metastasis of SCC in animal models.