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Recruitment of the SWI/SNF chromatin remodeling complex by transcriptional activators

Recruitment of the SWI/SNF chromatin remodeling complex by transcriptional activators

Genes and Development 13(18): 2369-2374

ISSN/ISBN: 0890-9369

PMID: 10500094

SWI/SNF is a chromatin remodeling complex that facilitates expression of a number of yeast genes. Here we demonstrate that SWI/SNE can be recruited from yeast nuclear extracts by a transcriptional activator. Recruitment is dependent on an activation domain but not on promoter sequences, TBP, or RNA polymerase II holoenzyme. We also show that acidic activation domains can target SWI/SNF remodeling activity. These results demonstrate that SWI/SNF activity can be targeted by gene-specific activators and that this recruitment can occur independently of Pol II holoenzyme. Reprinted by permission of the publisher.

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Accession: 009956680

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