+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Reduced macrophage infiltration and demyelination in mice lacking the chemokine receptor CCR5 following infection with a neurotropic coronavirus



Reduced macrophage infiltration and demyelination in mice lacking the chemokine receptor CCR5 following infection with a neurotropic coronavirus



Virology 288(1): 8-17



Studies were performed to investigate the contributions of the CC chemokine receptor CCR5 in host defense and disease development following intracranial infection with mouse hepatitis virus (MHV). T cell recruitment was impaired in MHV-infected CCR5(-/-) mice at day 7 postinfection (pi), which correlated with increased (P < or = 0.03) titers within the brain. However, by day 12 pi, T cell infiltration into the CNS of infected CCR5(-/-) and CCR5(+/+) mice was similar and both strains exhibited comparable viral titers, indicating that CCR5 expression is not essential for host defense. Following MHV infection of CCR5(+/+) mice, greater than 50% of cells expressing CCR5 antigen were activated macrophage/microglia (determined by F4/80 antigen expression). In addition, infected CCR5(-/-) mice exhibited reduced (P < or = 0.02) macrophage (CD45(high)F4/80(+)) infiltration, which correlated with a significant reduction (P < or = 0.001) in the severity of demyelination compared to CCR5(+/+) mice. These data indicate that CCR5 contributes to MHV-induced demyelination by allowing macrophages to traffic into the CNS.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 009957200

Download citation: RISBibTeXText

PMID: 11543653

DOI: 10.1006/viro.2001.1050


Related references

Macrophage infiltration, but not apoptosis, is correlated with immune-mediated demyelination following murine infection with a neurotropic coronavirus. Journal of Virology 73(10): 8771-8780, 1999

The dual CCR2/CCR5 chemokine receptor antagonist Cenicriviroc reduces macrophage infiltration and disease severity in Duchenne muscular dystrophy (Dmdmdx-4Cv) mice. Plos one 13(3): E0194421, 2018

Increased brain damage after ischaemic stroke in mice lacking the chemokine receptor CCR5. British Journal of Pharmacology 160(2): 311-321, 2010

Low expression of chemokine receptor CCR5 in human colorectal cancer correlates with lymphatic dissemination and reduced CD8+ T-cell infiltration. International Journal of Colorectal Disease 25(4): 417-424, 2010

Functional analysis of the CC chemokine receptor 5 (CCR5) on virus-specific CD8+ T cells following coronavirus infection of the central nervous system. Virology 312(2): 7-14, 2003

Bystander CD4 T cells do not mediate demyelination in mice infected with a neurotropic coronavirus. Journal of Neuroimmunology 137(1-2): 42-50, 2003

A novel chemokine-receptor-5 (CCR5) blocker, SCH532706, has differential effects on CCR5+CD4+ and CCR5+CD8+ T cell numbers in chronic HIV infection. Aids Research and Human Retroviruses 26(6): 653-661, 2010

Demyelination is mediated by gamma-delta T cells in mice infected with a neurotropic coronavirus. Journal of Neurovirology 8(Suppl. 1): 31, 2002

Mice Deficient in the Chemokine Receptor CXCR2 Have Reduced Neutrophil Infiltration but Not Reduced Neovascularization Following Corneal Injury. ARVO Annual Meeting Abstract Search and Program Planner : Abstract No 1746, 2002

Cutting edge: CD8 T cell-mediated demyelination is IFN-c dependent in mice infected with a neurotropic coronavirus. Journal of Immunology 168(4): 47-51, 2002

Depletion of blood-borne macrophages does not reduce demyelination in mice infected with a neurotropic coronavirus. Journal of Virology 73(8): 6327-6334, 1999

Interplay of complement and Fc receptors in antibody-induced demyelination in mice infected with a neurotropic coronavirus. FASEB Journal 18(4-5): Abst 778 3, 2004

Cutting Edge: Cd8 T Cell-Mediated Demyelination Is Ifn- Dependent in Mice Infected with a Neurotropic Coronavirus. The Journal of Immunology 168(4): 1547-1551, 2002

Cutting edge: CD8 T cell-mediated demyelination is IFN-gamma dependent in mice infected with a neurotropic coronavirus. Journal of Immunology 168(4): 1547-1551, 2002

Virus-specific antibody, in the absence of T cells, mediates demyelination in mice infected with a neurotropic coronavirus. American Journal of Pathology 166(3): 801-809, 2005